Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4713_RS04545 Genome accession   NZ_CP137103
Coordinates   848523..849464 (-) Length   313 a.a.
NCBI ID   WP_000249567.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 21334     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 843523..854464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4713_RS04505 comYH 844016..844969 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  R4713_RS04510 - 845030..845617 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  R4713_RS04515 comGG/cglG 845754..846167 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4713_RS04520 comGF/cglF 846145..846606 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4713_RS04525 comGE/cglE 846569..846871 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4713_RS04530 comGD/cglD 846834..847238 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4713_RS04535 comGC/cglC 847231..847557 (-) 327 WP_000738650.1 comG operon protein ComGC Machinery gene
  R4713_RS04540 comGB/cglB 847559..848575 (-) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4713_RS04545 comGA/cglA/cilD 848523..849464 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  R4713_RS04550 - 849540..849905 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4713_RS04555 - 850056..851114 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R4713_RS04560 nagA 851277..852428 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4713_RS04565 - 852581..854398 (-) 1818 WP_001220865.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35573.48 Da        Isoelectric Point: 6.0083

>NTDB_id=895341 R4713_RS04545 WP_000249567.1 848523..849464(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 21334]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=895341 R4713_RS04545 WP_000249567.1 848523..849464(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 21334]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGGCTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCATGCTAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTTTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361