Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R4713_RS04540 Genome accession   NZ_CP137103
Coordinates   847559..848575 (-) Length   338 a.a.
NCBI ID   WP_013193332.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 21334     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 842559..853575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4713_RS04495 - 842643..842774 (-) 132 WP_000768904.1 hypothetical protein -
  R4713_RS04500 - 842775..843965 (-) 1191 WP_081574829.1 acetate kinase -
  R4713_RS04505 comYH 844016..844969 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  R4713_RS04510 - 845030..845617 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  R4713_RS04515 comGG/cglG 845754..846167 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4713_RS04520 comGF/cglF 846145..846606 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4713_RS04525 comGE/cglE 846569..846871 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4713_RS04530 comGD/cglD 846834..847238 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4713_RS04535 comGC/cglC 847231..847557 (-) 327 WP_000738650.1 comG operon protein ComGC Machinery gene
  R4713_RS04540 comGB/cglB 847559..848575 (-) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4713_RS04545 comGA/cglA/cilD 848523..849464 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  R4713_RS04550 - 849540..849905 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4713_RS04555 - 850056..851114 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R4713_RS04560 nagA 851277..852428 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38524.60 Da        Isoelectric Point: 9.6162

>NTDB_id=895340 R4713_RS04540 WP_013193332.1 847559..848575(-) (comGB/cglB) [Streptococcus pneumoniae strain 21334]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=895340 R4713_RS04540 WP_013193332.1 847559..848575(-) (comGB/cglB) [Streptococcus pneumoniae strain 21334]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TAACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTTTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae D39

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae R6

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae TIGR4

99.112

100

0.991
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503