Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   R4713_RS04520 Genome accession   NZ_CP137103
Coordinates   846145..846606 (-) Length   153 a.a.
NCBI ID   WP_000250545.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 21334     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 841145..851606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4713_RS04485 - 841456..842280 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  R4713_RS04490 rnpA 842255..842626 (-) 372 WP_000739246.1 ribonuclease P protein component -
  R4713_RS04495 - 842643..842774 (-) 132 WP_000768904.1 hypothetical protein -
  R4713_RS04500 - 842775..843965 (-) 1191 WP_081574829.1 acetate kinase -
  R4713_RS04505 comYH 844016..844969 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  R4713_RS04510 - 845030..845617 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  R4713_RS04515 comGG/cglG 845754..846167 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4713_RS04520 comGF/cglF 846145..846606 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4713_RS04525 comGE/cglE 846569..846871 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4713_RS04530 comGD/cglD 846834..847238 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4713_RS04535 comGC/cglC 847231..847557 (-) 327 WP_000738650.1 comG operon protein ComGC Machinery gene
  R4713_RS04540 comGB/cglB 847559..848575 (-) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4713_RS04545 comGA/cglA/cilD 848523..849464 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  R4713_RS04550 - 849540..849905 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4713_RS04555 - 850056..851114 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17855.34 Da        Isoelectric Point: 6.4832

>NTDB_id=895336 R4713_RS04520 WP_000250545.1 846145..846606(-) (comGF/cglF) [Streptococcus pneumoniae strain 21334]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RFYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=895336 R4713_RS04520 WP_000250545.1 846145..846606(-) (comGF/cglF) [Streptococcus pneumoniae strain 21334]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
CGTCATCAGTGGAGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTGGAAGGCAAT
CGTTTCTATATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae D39

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae R6

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae TIGR4

93.464

100

0.935
  comGF/cglF Streptococcus mitis SK321

92.81

100

0.928
  comGF/cglF Streptococcus mitis NCTC 12261

91.503

100

0.915