Detailed information NTG0089520

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: LDO80_RS00105
• Genome accession: NZ_AP024933
• Coordinates: 19844..22507 (-)
• Length: 887 a.a.
• NCBI ID: WP_223965811.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain AA3-7
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 14844..27507

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDO80_RS00085 (TthAA37_00130)	-	15690..16322 (-)	633	WP_143584081.1	histidine phosphatase family protein	-
LDO80_RS00090 (TthAA37_00140)	purM	16319..17320 (-)	1002	WP_143584083.1	phosphoribosylformylglycinamidine cyclo-ligase	-
LDO80_RS00095 (TthAA37_00150)	gatB	17326..18735 (-)	1410	WP_143584085.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
LDO80_RS00100 (TthAA37_00160)	pilT	18746..19831 (-)	1086	WP_143584087.1	type IV pilus twitching motility protein PilT	Machinery gene
LDO80_RS00105 (TthAA37_00170)	pilF	19844..22507 (-)	2664	WP_223965811.1	type IV pilus assembly ATPase PilB	Machinery gene
LDO80_RS00110 (TthAA37_00180)	-	22504..22989 (-)	486	WP_014628818.1	YqeG family HAD IIIA-type phosphatase	-
LDO80_RS00115 (TthAA37_00190)	pgeF	23024..23752 (-)	729	WP_014628819.1	peptidoglycan editing factor PgeF	-
LDO80_RS00120 (TthAA37_00200)	surE	23749..24504 (-)	756	WP_224065386.1	5'/3'-nucleotidase SurE	-
LDO80_RS00125 (TthAA37_00210)	-	24563..24769 (-)	207	WP_143584091.1	cold-shock protein	-
LDO80_RS00130 (TthAA37_00220)	-	24869..25516 (-)	648	WP_223966705.1	transposase	-
LDO80_RS00135	-	25458..25643 (+)	186	WP_223966770.1	hypothetical protein	-
LDO80_RS00140 (TthAA37_00240)	-	25962..27251 (+)	1290	WP_014628822.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 97928.05 Da
• Isoelectric Point: 5.5424

>NTDB_id=89520 LDO80_RS00105 WP_223965811.1 19844..22507(-) (pilF) [Thermus thermophilus strain AA3-7]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKDLMLGEFLLKKGLISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAALLL
RSDALRYGAVPIGFQNGKVEVVLSDPRHKEAVAQLLNRPARFYLALPQAWEELFRRAYPQKNRLGEVLVQEGKLSREALK
EALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRRGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYVDPEEDPPD
PGASLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNHEVAPAVATEAAITKLIERFYGKAELS
EIAKEFAKKQEEEAPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVISV
VKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKPYG
IFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRDS
ETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGLSE
AEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTLEE
VLARTIE

Nucleotide

• Length: 2664 bp

>NTDB_id=89520 LDO80_RS00105 WP_223965811.1 19844..22507(-) (pilF) [Thermus thermophilus strain AA3-7]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAGAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTGGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCTTCCTGTACGCCCTGGCCAAACACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAGAAGGACCTCATGCTCGGGGAGTTCCTCCTGAAAAAGGGGCTGATCTCCCGCGAGGCCCTGGAGGAGGCCCT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CCTTGGCGGAGCAGAAGGGGCTAGAGTTTCTGGAAAGCACCGAGGGGATCGTCCCCGACCCCTCCGCCGCCCTCCTCCTC
CGCTCCGACGCCCTGCGCTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGAAGGTGGAGGTGGTCCTCTCCGACCCCCG
GCACAAGGAAGCGGTGGCCCAGCTCCTCAATCGGCCTGCCCGCTTCTACCTCGCCCTCCCCCAGGCCTGGGAGGAGCTCT
TCCGCCGGGCCTACCCGCAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAGGCGCTAAAG
GAAGCCCTGGAGGTGCAGAAGGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCGCCC
CGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCGGGGCGGGGGCCGGCTGGAGGACACGCTGGTCCAGTCGGGGAAGC
TCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGGAGGACCCCCCCGAT
CCCGGCGCCTCCCTCCTCCTCCCGGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGAAACCGCCT
CGTCCTCCTGATGAAGGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGAGGAAGGGCCTGAACC
ACGAGGTGGCCCCCGCTGTGGCCACGGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGTAAGGCCGAGCTCTCG
GAGATCGCCAAGGAGTTCGCCAAAAAGCAGGAGGAGGAGGCCCCAAGCCCCCTGGAGCTGGACGAGAGCGCCGCCCAGAA
GTTCGTGAAGCAGGTGATCCGGGAGGCCTTCCTCCAGGACGCCTCCGACATCCACATCGAGCCCAGGCAGAACGACGTCC
AGGTGCGCCTCCGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCCAAGGGGGCGCTGAACGCGGTGATCTCCGTG
GTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAACGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGGGGCCAT
CGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGGCCTCGG
ACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCCTACGGC
ATCTTCCTCATCACCGGGCCCACAGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCACCCCCGA
CAAGAACACCCAGACCATTGAGGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCGCAGGCGG
GCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGACTCC
GAGACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGCCGCCCA
GGCCATCACCCGCCTGGACGAGATGGGGGTGGAACCCTTCAACATCTCCGCCGCCCTCATCGGCGTCCTCTCCCAGCGCC
TGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTTTCCGAG
GCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCGCTACGC
CATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCAAGGAGA
TCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTCGAGGAG
GTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (511-777)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-301)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-478)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	98.875	100	0.991
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	55.955	100	0.561