Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   LDO10_RS06655 Genome accession   NZ_AP024929
Coordinates   1233819..1234460 (+) Length   213 a.a.
NCBI ID   WP_223970918.1    Uniprot ID   -
Organism   Thermus thermophilus strain AA2-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1228819..1239460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO10_RS06630 (TthAA22_12990) ilvN 1229238..1229750 (-) 513 WP_223965549.1 acetolactate synthase small subunit -
  LDO10_RS06635 (TthAA22_13000) ilvB 1229747..1231435 (-) 1689 WP_223965550.1 biosynthetic-type acetolactate synthase large subunit -
  LDO10_RS06640 (TthAA22_13010) - 1231591..1232571 (-) 981 WP_223970907.1 YpdA family putative bacillithiol disulfide reductase -
  LDO10_RS06645 (TthAA22_13020) pilA/pilA1 1232737..1233207 (+) 471 WP_223970911.1 GspH/FimT family protein Machinery gene
  LDO10_RS06650 (TthAA22_13030) pilA/pilA2 1233262..1233822 (+) 561 WP_267461438.1 type II secretion system protein Machinery gene
  LDO10_RS06655 (TthAA22_13040) pilA/pilA3 1233819..1234460 (+) 642 WP_223970918.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LDO10_RS06660 (TthAA22_13050) - 1234428..1235528 (-) 1101 WP_223970920.1 transposase -
  LDO10_RS06665 (TthAA22_13070) comZ 1235727..1237403 (+) 1677 WP_223970923.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  LDO10_RS13460 - 1237621..1237731 (+) 111 Protein_1295 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LDO10_RS06675 (TthAA22_13090) - 1238414..1238794 (+) 381 WP_223968480.1 DUF1640 domain-containing protein -
  LDO10_RS06680 (TthAA22_13100) - 1238870..1239412 (+) 543 WP_223968482.1 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 22737.69 Da        Isoelectric Point: 6.2966

>NTDB_id=89506 LDO10_RS06655 WP_223970918.1 1233819..1234460(+) (pilA/pilA3) [Thermus thermophilus strain AA2-2]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGDQNQAFSLPL
PPLSGSDGGPKDTLTLYYVTSLRDLASACRRVDYGFEGDTLRRSDVSATPTSGSDCTTPSPNYQPLAEGILALDIQYQCS
DSTTRNTPDCGANAYPRSAKVTVAGYSLTPVTNPGPASLTTVTSKLAPPAYPA

Nucleotide


Download         Length: 642 bp        

>NTDB_id=89506 LDO10_RS06655 WP_223970918.1 1233819..1234460(+) (pilA/pilA3) [Thermus thermophilus strain AA2-2]
ATGAAGCGCGGCTTCACCCTGGTGGAGGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACAAGCGAACTCGCCCGGAACACCCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGGGCCCGCTACTGGAATAGTGGCGACCAAAACCAAGCCTTCAGCCTCCCCCTT
CCCCCGCTTTCGGGAAGCGACGGAGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGAAGGGGACACCTTGCGTCGGAGCGACGTGAGCGCCACACCCACCTCGGGGA
GCGACTGCACCACCCCTTCCCCCAACTACCAACCCCTGGCCGAGGGCATCCTGGCCCTGGACATCCAATACCAGTGCAGC
GACAGCACCACCCGAAACACTCCGGACTGCGGCGCAAACGCCTACCCCCGCTCGGCCAAGGTTACGGTGGCGGGCTACTC
CCTGACCCCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCTCAAAACTTGCACCTCCCGCATACCCCGCAT
AA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

91.707

96.244

0.883

Multiple sequence alignment