Detailed information NTG0894696

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: AAVC70_RS12300
• Genome accession: NZ_CP154898
• Coordinates: 2184999..2186120 (+)
• Length: 373 a.a.
• NCBI ID: WP_013350919.1
• Uniprot ID: A0A9P1NGF1
• Organism: Bacillus amyloliquefaciens strain Fad 97
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 2179999..2191120

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVC70_RS12280 (AAVC70_12280)	-	2180319..2181314 (-)	996	WP_013350915.1	DUF5105 domain-containing protein	-
AAVC70_RS12285 (AAVC70_12285)	-	2181431..2182829 (+)	1399	Protein_2261	YcdB/YcdC domain-containing protein	-
AAVC70_RS12290 (AAVC70_12290)	-	2183031..2184389 (+)	1359	WP_014469699.1	YcdB/YcdC domain-containing protein	-
AAVC70_RS12295 (AAVC70_12295)	-	2184386..2184889 (-)	504	WP_014469700.1	M15 family metallopeptidase	-
AAVC70_RS12300 (AAVC70_12300)	rapF	2184999..2186120 (+)	1122	WP_013350919.1	tetratricopeptide repeat protein	Regulator
AAVC70_RS12305 (AAVC70_12305)	-	2186234..2187013 (+)	780	WP_013350920.1	glucose 1-dehydrogenase	-
AAVC70_RS12310 (AAVC70_12310)	-	2187097..2188776 (+)	1680	WP_345808512.1	alpha-glucosidase	-
AAVC70_RS12315 (AAVC70_12315)	-	2188761..2189255 (-)	495	WP_013350922.1	DUF4188 domain-containing protein	-
AAVC70_RS12320 (AAVC70_12320)	-	2189256..2189801 (-)	546	WP_013350923.1	PadR family transcriptional regulator	-
AAVC70_RS12325 (AAVC70_12325)	-	2190010..2190969 (+)	960	WP_013350924.1	metal ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44347.46 Da
• Isoelectric Point: 5.0035

>NTDB_id=894696 AAVC70_RS12300 WP_013350919.1 2184999..2186120(+) (rapF) [Bacillus amyloliquefaciens strain Fad 97]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHEKALEYLECALEHAERLDDHYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFQQVIQAVFSLTHIYFKTERYSDAAASYARGMKSAAEWEDEIYMTKFRLIKELYQGDGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=894696 AAVC70_RS12300 WP_013350919.1 2184999..2186120(+) (rapF) [Bacillus amyloliquefaciens strain Fad 97]
ATGAAATCCAAGATTCCATCTGAAGAAGTAGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTATTGTATTTTT
CATTGATGGAATTTAGGCATCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAACGGGCTGAGGAAAAACTAGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAAGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGTAACCTGATGGACGCCCAGCAGCATGAAAAAGCGCTGGAATACCTTGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCACTATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTTTGGGTGACTTTTGCAAGGCGGCG
GAAGACTTTGAACGAGCGGCTGCCGGTTTTGAAGCGCACAGCTTCCAGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAGATACAGTGACGCCGCCGCCAGCTATGCGCGGGGCATGAAAAGCGCGGCGGAGTGGGAAG
ACGAGATCTATATGACAAAATTCCGTCTGATTAAGGAGCTTTATCAGGGTGACGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAGGAGCTGTTGGCCGACACTGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAATTACGCAT
AA

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (187-251)

3D structure

• AlphaFold DB: A0A9P1NGF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402