Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   R3L12_RS15115 Genome accession   NZ_CP137057
Coordinates   3236211..3236969 (+) Length   252 a.a.
NCBI ID   WP_025095393.1    Uniprot ID   A0AB38Z0B0
Organism   Acinetobacter soli strain SNUBHAB0075     
Function   assists with secretin assembly and localization (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3231211..3241969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3L12_RS15090 (R3L12_15070) - 3232113..3233396 (+) 1284 WP_149929728.1 glycosyltransferase family 1 protein -
  R3L12_RS15095 (R3L12_15075) - 3233422..3233973 (+) 552 WP_149929727.1 phosphatase PAP2 family protein -
  R3L12_RS15100 (R3L12_15080) iscX 3234045..3234245 (+) 201 WP_149929726.1 Fe-S cluster assembly protein IscX -
  R3L12_RS15105 (R3L12_15085) ndk 3234354..3234785 (+) 432 WP_004932912.1 nucleoside-diphosphate kinase -
  R3L12_RS15110 (R3L12_15090) rlmN 3234907..3236151 (+) 1245 WP_004932914.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  R3L12_RS15115 (R3L12_15095) pilF 3236211..3236969 (+) 759 WP_025095393.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  R3L12_RS15120 (R3L12_15100) - 3236960..3237742 (+) 783 WP_004943670.1 DUF4115 domain-containing protein -
  R3L12_RS15125 (R3L12_15105) ispG 3237768..3238883 (+) 1116 WP_004932923.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  R3L12_RS15130 (R3L12_15110) hisS 3238880..3240172 (+) 1293 WP_149929725.1 histidine--tRNA ligase -
  R3L12_RS15135 (R3L12_15115) - 3240204..3240905 (+) 702 WP_004932928.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 252 a.a.        Molecular weight: 28565.55 Da        Isoelectric Point: 9.4949

>NTDB_id=894495 R3L12_RS15115 WP_025095393.1 3236211..3236969(+) (pilF) [Acinetobacter soli strain SNUBHAB0075]
MLAALLLSGCQTTSIKKDPQKGAKVRTQLAAEYIKTGDLDAAKRALDHALKVEPKDSNANMMMGVLLQQEGSRPNLEKAD
EYFARAVALDPENAQARNNYGAYLYQMKRYHDAIQQLTRAGSTLGYDQRYLALENLGRIYLHLGGVSNAEKMFKQALQAN
RDSTVSMLELAEIYYLQHKIPASTEMYEQYVRTVGQKNQSARALWVGIRVARANADRTGVQVLVNQLRIFYPDSPEYQRY
MQLQYSTEAVWK

Nucleotide


Download         Length: 759 bp        

>NTDB_id=894495 R3L12_RS15115 WP_025095393.1 3236211..3236969(+) (pilF) [Acinetobacter soli strain SNUBHAB0075]
ATGCTGGCGGCGCTTCTTTTATCTGGATGCCAAACGACTTCAATAAAAAAAGATCCGCAAAAAGGCGCGAAAGTCCGGAC
TCAACTTGCAGCCGAATATATCAAAACAGGTGATCTTGATGCTGCCAAGCGTGCGCTAGATCATGCACTCAAAGTCGAAC
CGAAAGACAGCAATGCCAACATGATGATGGGGGTGCTGTTGCAGCAAGAGGGCAGTCGCCCAAATCTTGAAAAAGCCGAT
GAATATTTTGCACGTGCTGTAGCGCTGGATCCGGAGAATGCTCAGGCCAGAAACAACTATGGCGCTTATTTGTATCAGAT
GAAACGTTATCATGATGCAATTCAACAATTGACACGCGCCGGTAGTACATTAGGATATGACCAGCGCTATCTGGCACTGG
AAAATTTAGGTCGGATTTATTTGCATCTGGGGGGTGTTTCGAATGCTGAAAAAATGTTCAAACAGGCACTTCAGGCAAAC
CGTGATTCAACTGTTTCAATGTTAGAGTTAGCAGAAATTTATTATTTGCAACACAAAATTCCTGCCTCGACAGAGATGTA
TGAACAATATGTTCGAACTGTTGGGCAGAAAAATCAAAGTGCTCGTGCACTTTGGGTGGGAATTCGCGTCGCGAGAGCGA
ATGCCGACCGGACTGGGGTGCAAGTATTGGTAAATCAGCTTCGTATTTTTTACCCAGATAGTCCAGAATATCAACGTTAT
ATGCAATTACAGTACAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_10)

(132-161)

Tetratricopeptide repeat (TPR_8)

(23-56)

Tetratricopeptide repeat (TPR_16)

(76-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baylyi ADP1

85.317

100

0.853
  pilF Acinetobacter baumannii D1279779

74.8

99.206

0.742