Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   R0Q54_RS12470 Genome accession   NZ_CP136992
Coordinates   2331368..2331817 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain J46     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2326368..2336817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0Q54_RS12430 (R0Q54_12430) - 2326693..2326890 (+) 198 WP_121509395.1 hypothetical protein -
  R0Q54_RS12435 (R0Q54_12435) yckC 2326910..2327365 (+) 456 WP_014478793.1 RDD family protein -
  R0Q54_RS12440 (R0Q54_12440) yckD 2327447..2327779 (+) 333 WP_014478794.1 YckD family protein -
  R0Q54_RS12445 (R0Q54_12445) - 2327907..2328329 (+) 423 WP_014478795.1 MarR family winged helix-turn-helix transcriptional regulator -
  R0Q54_RS12450 (R0Q54_12450) - 2328317..2328637 (+) 321 WP_014478796.1 DUF3147 family protein -
  R0Q54_RS12455 (R0Q54_12455) - 2328656..2329400 (+) 745 Protein_2409 AAA family ATPase -
  R0Q54_RS12460 (R0Q54_12460) bglC 2329473..2330906 (+) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  R0Q54_RS12465 (R0Q54_12465) nin/comJ 2330943..2331341 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  R0Q54_RS12470 (R0Q54_12470) nucA/comI 2331368..2331817 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  R0Q54_RS12475 (R0Q54_12475) tlpC 2331985..2333706 (-) 1722 Protein_2413 methyl-accepting chemotaxis protein TlpC -
  R0Q54_RS12480 (R0Q54_12480) hxlB 2333817..2334374 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  R0Q54_RS12485 (R0Q54_12485) hxlA 2334380..2335012 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  R0Q54_RS12490 (R0Q54_12490) hxlR 2335241..2335603 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=894058 R0Q54_RS12470 WP_014475770.1 2331368..2331817(-) (nucA/comI) [Bacillus subtilis strain J46]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=894058 R0Q54_RS12470 WP_014475770.1 2331368..2331817(-) (nucA/comI) [Bacillus subtilis strain J46]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987