Detailed information NTG0893949

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R3J29_RS05560
• Genome accession: NZ_CP136974
• Coordinates: 1271112..1272242 (+)
• Length: 376 a.a.
• NCBI ID: WP_071869741.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain CFBP8075
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1266112..1277242

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R3J29_RS05540 (R3J29_05535)	-	1266391..1267215 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
R3J29_RS05545 (R3J29_05540)	-	1267845..1268672 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
R3J29_RS05550 (R3J29_05545)	-	1269057..1269269 (-)	213	WP_071869740.1	hypothetical protein	-
R3J29_RS05555 (R3J29_05550)	pilT	1269912..1270946 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
R3J29_RS05560 (R3J29_05555)	pilU	1271112..1272242 (+)	1131	WP_071869741.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R3J29_RS05565 (R3J29_05560)	-	1272935..1273903 (-)	969	WP_071869748.1	YafY family protein	-
R3J29_RS05570 (R3J29_05565)	-	1274686..1275495 (+)	810	WP_021358437.1	YdiY family protein	-
R3J29_RS05575 (R3J29_05570)	hemC	1275549..1276466 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
R3J29_RS05580 (R3J29_05575)	-	1276474..1277232 (-)	759	WP_027700753.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42506.76 Da
• Isoelectric Point: 6.5563

>NTDB_id=893949 R3J29_RS05560 WP_071869741.1 1271112..1272242(+) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8075]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHNGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=893949 R3J29_RS05560 WP_071869741.1 1271112..1272242(+) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8075]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAACGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	50.843	94.681	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402