Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAG092_RS15035 Genome accession   NZ_CP154874
Coordinates   3116826..3118328 (-) Length   500 a.a.
NCBI ID   WP_373387296.1    Uniprot ID   -
Organism   Pseudomonas alcaligenes strain Med1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3111826..3123328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG092_RS15005 (AAG092_14930) yjgA 3112715..3113236 (-) 522 WP_110683806.1 ribosome biogenesis factor YjgA -
  AAG092_RS15010 (AAG092_14935) pmbA 3113326..3114672 (+) 1347 WP_373387292.1 metalloprotease PmbA -
  AAG092_RS15015 (AAG092_14940) - 3114727..3114999 (-) 273 WP_373387293.1 HPr family phosphocarrier protein -
  AAG092_RS15020 (AAG092_14945) rapZ 3115008..3115865 (-) 858 WP_373387294.1 RNase adapter RapZ -
  AAG092_RS15025 (AAG092_14950) ptsN 3115882..3116346 (-) 465 WP_373387295.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAG092_RS15030 (AAG092_14955) hpf 3116360..3116668 (-) 309 WP_110683811.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AAG092_RS15035 (AAG092_14960) rpoN/rpoN1 3116826..3118328 (-) 1503 WP_373387296.1 RNA polymerase factor sigma-54 Machinery gene
  AAG092_RS15040 (AAG092_14965) lptB 3118469..3119194 (-) 726 WP_110683813.1 LPS export ABC transporter ATP-binding protein -
  AAG092_RS15045 (AAG092_14970) lptA 3119194..3119736 (-) 543 WP_203788485.1 lipopolysaccharide transport periplasmic protein LptA -
  AAG092_RS15050 (AAG092_14975) lptC 3119723..3120289 (-) 567 WP_373387297.1 LPS export ABC transporter periplasmic protein LptC -
  AAG092_RS15055 (AAG092_14980) kdsC 3120299..3120823 (-) 525 WP_373387298.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AAG092_RS15060 (AAG092_14985) - 3120824..3121798 (-) 975 WP_373387299.1 KpsF/GutQ family sugar-phosphate isomerase -
  AAG092_RS15065 (AAG092_14990) - 3122048..3122857 (+) 810 WP_110683817.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 56427.27 Da        Isoelectric Point: 4.4643

>NTDB_id=893907 AAG092_RS15035 WP_373387296.1 3116826..3118328(-) (rpoN/rpoN1) [Pseudomonas alcaligenes strain Med1]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGEDFDNSDPMADGAEQNTQAQQDSSSFQ
DSTVAVNNTEGGEDGEWHDRIPSELPVDTAWEDVYQTSASSLPSSDDDEWDFTTRTSAGESLQSHLLWQLNLVPMSDTDK
LIGVTIIDCINDQGYLEESIDEIVESFDPQLDIEADEVEAVLHRIQHFEPAGVGARDLRECLLLQLRQLPADTPWLAEAQ
RLASEHLDVLGGRDYSQLMRRMKLKEDELRQVIELIQSLNPRPGSQIEASEPEYVVPDVIVRKHNERWLVELNQEAVPRL
RVNPQYAGFVKRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMH
ESTISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRT
VAKYRESLGIAPSSERKRLM

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=893907 AAG092_RS15035 WP_373387296.1 3116826..3118328(-) (rpoN/rpoN1) [Pseudomonas alcaligenes strain Med1]
ATGAAACCGTCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACTCCGCAGCTGCAACAGGCCATCCGACTCCTCCA
GCTCTCCACCCTGGATCTGCAGCAGGAAATCCAGGAAGCCCTGGAATCCAACCCCATGCTGGAGCGCCAGGAAGACGGCG
AGGATTTCGACAACTCCGACCCCATGGCCGACGGCGCCGAGCAGAACACCCAGGCGCAGCAGGACAGCTCCAGCTTCCAG
GACAGCACCGTCGCGGTGAACAACACCGAGGGTGGCGAGGACGGCGAGTGGCACGACCGCATCCCCAGCGAGCTGCCGGT
CGATACCGCCTGGGAAGACGTCTACCAGACCAGCGCCAGCAGCCTGCCCTCCAGCGATGACGACGAGTGGGACTTCACCA
CCCGCACCTCGGCCGGCGAGAGCCTGCAGAGCCACCTGCTGTGGCAGCTCAACCTGGTGCCGATGTCCGACACCGACAAG
CTGATCGGCGTCACCATCATCGACTGCATCAACGACCAGGGTTACCTGGAGGAATCCATCGACGAAATCGTCGAGTCCTT
CGACCCGCAGCTGGACATCGAGGCCGACGAAGTCGAGGCCGTGCTGCACCGCATCCAGCACTTCGAGCCCGCCGGCGTCG
GCGCACGTGACCTGCGCGAATGCCTGCTCCTGCAACTGCGCCAGCTACCCGCCGACACGCCCTGGCTGGCCGAGGCGCAG
CGCCTGGCCAGCGAGCATCTGGATGTGCTCGGCGGCCGCGACTACAGCCAGCTGATGCGCCGCATGAAGCTGAAAGAGGA
CGAACTGCGCCAGGTCATCGAGCTGATCCAGAGCCTCAACCCGCGCCCCGGCTCGCAGATCGAGGCCAGCGAGCCCGAGT
ACGTGGTGCCCGACGTCATCGTGCGCAAGCACAACGAGCGCTGGCTGGTGGAGCTGAACCAGGAGGCAGTGCCGCGCCTG
CGCGTCAACCCGCAGTATGCCGGCTTCGTCAAACGCGCCGACTCCAGCGCCGACAACACCTTCATGCGCAACCAGCTGCA
GGAGGCGCGCTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAGACGCTGATGAAGGTGGCGACGCAGATCGTCGAGCACC
AGCGCGGCTTCCTCGAATACGGCGACGAGGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAGGCGGTGGGCATGCAC
GAGTCGACCATCTCGCGGGTCACCACGCAGAAATTCATGCACACCCCGCGCGGCATCTACGAGCTGAAATACTTCTTCTC
CAGCCACGTCAGCACCTCCGAGGGCGGCGAATGCTCGTCCACCGCGATCCGCGCCATCATCAAGAAACTGGTGGCCGCGG
AAAACGCGAAAAAGCCGTTGAGCGACAGCAAGATCGCTGGTTTACTGGAAGCACAGGGCATTCAGGTAGCCCGCCGCACC
GTCGCCAAGTACCGCGAGTCCCTCGGCATCGCGCCGTCCAGCGAACGCAAGCGACTGATGTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(340-498)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.168

100

0.458