Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AAG092_RS08350 Genome accession   NZ_CP154874
Coordinates   1719715..1721934 (+) Length   739 a.a.
NCBI ID   WP_373389314.1    Uniprot ID   -
Organism   Pseudomonas alcaligenes strain Med1     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1714715..1726934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG092_RS08325 (AAG092_08270) - 1714926..1715612 (+) 687 WP_373389311.1 OmpW family protein -
  AAG092_RS08330 (AAG092_08275) - 1715738..1717006 (-) 1269 WP_110683454.1 malic enzyme-like NAD(P)-binding protein -
  AAG092_RS08335 (AAG092_08280) - 1717094..1718533 (-) 1440 WP_373389312.1 M48 family metalloprotease -
  AAG092_RS08340 (AAG092_08285) - 1718530..1719333 (-) 804 WP_373389313.1 thermonuclease family protein -
  AAG092_RS08345 (AAG092_08290) rpmE 1719339..1719551 (-) 213 WP_110683452.1 50S ribosomal protein L31 -
  AAG092_RS08350 (AAG092_08295) priA 1719715..1721934 (+) 2220 WP_373389314.1 primosomal protein N' Machinery gene
  AAG092_RS08355 (AAG092_08300) argS 1722113..1723852 (+) 1740 WP_373389315.1 arginine--tRNA ligase -
  AAG092_RS08360 (AAG092_08305) - 1723853..1724542 (+) 690 WP_373389316.1 SPOR domain-containing protein -
  AAG092_RS08365 (AAG092_08310) hslV 1724640..1725170 (+) 531 WP_068824557.1 ATP-dependent protease subunit HslV -
  AAG092_RS08370 (AAG092_08315) hslU 1725194..1726534 (+) 1341 WP_110683447.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81627.29 Da        Isoelectric Point: 8.9257

>NTDB_id=893870 AAG092_RS08350 WP_373389314.1 1719715..1721934(+) (priA) [Pseudomonas alcaligenes strain Med1]
MPDTILRLALPSPLRRLFDYLAPHGVPRTALQPGVRLRVPFGRREVVGVLVEVAQHSEVPADKLKPALQLLDRTPPLPAP
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGEPAEARQERYWHAVEGASADDPRLARAPRQREALRALAQHQHGVPHALL
SKLQLNKDSLDLLLDKGLVRVEVRRSAPLTRHGGWLAQPELPLNPEQRAAFHAVQAGMGGFGAYLLYGVTGSGKTEVYLQ
LIRHCLEAGKQALVLIPEINLGPQTVERFARRFNARIALLHSGVGDRERLDAWLAARDGEADIVIGTRSALFTPLKNPGL
IVVDEEHDASYKQQEGLRYHARDLALVRARQENLPIVLGSATPSLESLHNAHAGRYALLKLGQRAGNAKPPRFLRLDVKS
LPLDAGISRPLQQAIGQTLEAGQQVLVFLNRRGFAPTLLCHDCGWISQCPRCDARMTLHQRHHELRCHHCGHVERTPHNC
PDCQKVDLRPVGAGTERAEERLGILFPQFPVLRIDRDSTARKGAMDKLFATINQGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQLIVQVAGRAGRAEEPGKVIIQSHLADHPLLVQLTEQGFPAFAEQALSERRGAGLPPFAHL
ALLRGEAHKPGQAEAFLDDACTYAEQLCAELKLSGIELLGPVPAPMERRAGRFRAQLLLQCASRAPLHRLLAHWLPILEA
MPAGRAVRWSLDVDPIDLF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=893870 AAG092_RS08350 WP_373389314.1 1719715..1721934(+) (priA) [Pseudomonas alcaligenes strain Med1]
GTGCCCGACACCATCCTGCGCCTCGCCCTGCCCTCGCCGCTGCGCCGCCTGTTCGACTACCTGGCGCCGCATGGGGTGCC
GCGCACCGCGCTGCAGCCGGGGGTACGGCTGCGCGTGCCGTTCGGCCGCCGCGAGGTGGTAGGCGTGCTGGTGGAGGTGG
CGCAGCACAGCGAGGTGCCGGCGGACAAGCTCAAGCCGGCCCTGCAGCTGCTCGACCGCACGCCGCCACTGCCGGCGCCG
CTGTTCAAGCTGTGCCTGTGGACGGCGCAGTACTACCAGCACAGCCTCGGCGACACCCTCAGCTGGGCCCTGCCGGTGCT
GCTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGGAGCGCTACTGGCATGCCGTGGAAGGCGCCAGCGCCGACGATCCGC
GCCTGGCCCGCGCGCCGCGCCAGCGCGAGGCCCTGCGCGCCCTGGCGCAGCACCAGCACGGCGTGCCGCACGCCTTGCTG
AGCAAGCTGCAGCTGAACAAGGACAGCCTCGACCTGCTGCTGGACAAGGGCCTGGTGCGCGTCGAGGTACGCCGCAGCGC
GCCGCTCACCCGCCATGGCGGCTGGCTGGCGCAGCCGGAGCTGCCGCTCAACCCCGAGCAGCGCGCCGCCTTCCACGCCG
TGCAGGCCGGCATGGGCGGTTTCGGCGCCTACCTGCTGTACGGCGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAG
CTGATCCGCCACTGCCTGGAAGCCGGCAAGCAGGCCCTGGTGCTGATCCCCGAGATCAACCTGGGGCCGCAGACGGTGGA
ACGCTTCGCCCGCCGCTTCAATGCGCGCATCGCCCTGCTGCACTCCGGCGTGGGCGACCGCGAGCGCCTGGATGCCTGGC
TGGCAGCGCGCGACGGCGAGGCCGACATCGTCATCGGCACCCGTTCCGCACTGTTCACCCCGCTGAAGAACCCCGGGCTG
ATCGTGGTCGACGAGGAACACGACGCCTCCTATAAACAGCAGGAAGGCCTGCGCTACCACGCCCGCGACCTGGCCCTGGT
GCGCGCGCGCCAGGAGAACCTGCCGATCGTGCTGGGCTCGGCCACCCCCTCGCTGGAGAGCCTGCACAACGCCCACGCCG
GCCGCTACGCCCTGCTCAAGCTCGGCCAGCGCGCCGGCAACGCCAAGCCACCGCGCTTCCTGCGCCTGGACGTGAAGAGC
CTGCCGCTCGACGCCGGCATCTCGCGCCCGCTGCAGCAGGCCATCGGCCAGACCCTGGAGGCCGGCCAGCAGGTGCTGGT
GTTCCTCAACCGCCGCGGCTTCGCCCCCACCCTGCTGTGCCACGACTGCGGCTGGATCAGCCAGTGCCCGCGCTGCGACG
CGCGCATGACCCTGCACCAGCGCCACCACGAGCTGCGCTGCCACCACTGCGGCCATGTCGAGCGCACGCCACACAACTGC
CCGGACTGCCAGAAGGTCGACCTGCGCCCGGTCGGCGCCGGCACCGAGCGCGCCGAGGAGCGCCTGGGCATCCTCTTCCC
GCAGTTCCCGGTGCTGCGCATCGACCGCGACAGCACGGCGCGCAAGGGCGCCATGGACAAGCTGTTCGCCACCATCAACC
AGGGCGAGCCGTGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGCCACCACTTCCCGCGCGTGACCCTGGTCGCCATC
CTCGACGCCGACGGCGGCCTGTTCTCCGCCGACTTCCGCGCCAGCGAGCGCATGGCCCAGCTGATCGTCCAGGTCGCCGG
GCGCGCCGGGCGTGCCGAGGAGCCGGGCAAGGTGATCATCCAGAGCCACCTGGCCGACCATCCGCTGCTGGTCCAGCTCA
CCGAGCAAGGCTTCCCGGCCTTCGCCGAGCAGGCCCTGAGCGAGCGCCGCGGTGCCGGCCTGCCGCCTTTCGCCCACCTC
GCCCTGCTGCGCGGCGAGGCGCACAAGCCGGGCCAGGCCGAGGCCTTCCTCGACGACGCCTGCACCTATGCCGAGCAGCT
GTGTGCGGAGCTCAAGCTGAGCGGCATCGAGCTGCTCGGCCCGGTGCCGGCGCCGATGGAGCGCCGCGCCGGGCGTTTCC
GCGCCCAGCTGCTGCTGCAGTGCGCCAGCCGCGCGCCGCTGCACAGGCTGCTGGCGCACTGGCTGCCGATTCTCGAAGCC
ATGCCGGCCGGCCGCGCGGTGCGCTGGTCGCTGGATGTCGACCCCATCGACCTGTTCTGA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

Helicase conserved C-terminal domain (Helicase_C)

(522-592)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

45.71

100

0.461