Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   P4826_RS14720 Genome accession   NZ_CP136921
Coordinates   3076273..3077817 (+) Length   514 a.a.
NCBI ID   WP_317701129.1    Uniprot ID   -
Organism   Diaphorobacter limosus strain Y-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3071273..3082817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4826_RS14695 (P4826_14695) argS 3071661..3073340 (+) 1680 WP_317701124.1 arginine--tRNA ligase -
  P4826_RS14700 (P4826_14700) - 3073353..3073982 (+) 630 WP_317701125.1 SPOR domain-containing protein -
  P4826_RS14705 (P4826_14705) - 3074042..3074674 (+) 633 WP_317701126.1 thiol:disulfide interchange protein DsbA/DsbL -
  P4826_RS14710 (P4826_14710) lptA 3074791..3075432 (+) 642 WP_317701127.1 lipopolysaccharide transport periplasmic protein LptA -
  P4826_RS14715 (P4826_14715) lptB 3075441..3076229 (+) 789 WP_317701128.1 LPS export ABC transporter ATP-binding protein -
  P4826_RS14720 (P4826_14720) rpoN/rpoN1 3076273..3077817 (+) 1545 WP_317701129.1 RNA polymerase factor sigma-54 Machinery gene
  P4826_RS14725 (P4826_14725) - 3077837..3078274 (+) 438 WP_317701130.1 thioesterase family protein -
  P4826_RS14730 (P4826_14730) - 3078281..3078943 (-) 663 WP_317701131.1 PEP-CTERM sorting domain-containing protein -
  P4826_RS14735 (P4826_14735) - 3079078..3080901 (-) 1824 WP_317701132.1 methyl-accepting chemotaxis protein -
  P4826_RS14740 (P4826_14740) - 3081014..3082294 (+) 1281 WP_317701133.1 THUMP domain-containing protein -
  P4826_RS14745 (P4826_14745) - 3082284..3082766 (+) 483 WP_317701134.1 putative toxin-antitoxin system toxin component, PIN family -

Sequence

Protein


Download         Length: 514 a.a.        Molecular weight: 56193.96 Da        Isoelectric Point: 4.5419

>NTDB_id=893442 P4826_RS14720 WP_317701129.1 3076273..3077817(+) (rpoN/rpoN1) [Diaphorobacter limosus strain Y-1]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDDNPFLELASDDGTHEPEIHEAQQPIDNATYSEAASADA
EGAGAENDAETSAVAEPSDWDGDGSMDAAVEDGEWGSDGIAPASGAHDGDDTEVSELARSHESLTDFLHRQALSLRLSDV
DMAALRFLIESLNDDGYLDEPLESLAASLAGADDPEQLEELVHRFTVALRLLQNLEPTGVGARDLAECLTLQLHAMNRDE
EGDPAVIQTALAICSQPLDLLARRDLRRLAQATGSSDDDTRAAMALIARLEPRPGRRFADVERNVIAPDVIVKKTGRGAQ
QDFSVQLNPDVMPRLRVHEIYASALRGARGGDGHQALQSRLQEARWFIKNIQQRFDTILRVSRAIVERQKSFFVHGELAM
RPLVLREIADELGLHESTISRVTSAKYMATPQGTFELKYFFGSGLGTETGGNASSTAVRALIKQFVAAEDVKKPLSDNQI
AEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1545 bp        

>NTDB_id=893442 P4826_RS14720 WP_317701129.1 3076273..3077817(+) (rpoN/rpoN1) [Diaphorobacter limosus strain Y-1]
ATGAAGCCCGGCCTGTCACTGCGCGTTTCGCAGCATCTGGCGCTTACGCCGCAGTTGCAGCAATCGATTCGCCTGCTGCA
GCTGTCCACCCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTCGATGACAACCCGTTTCTGGAGCTGGCGTCCGACGATG
GGACGCATGAGCCTGAGATCCATGAAGCGCAGCAACCGATTGACAATGCTACATATTCAGAAGCTGCTAGCGCTGATGCA
GAGGGCGCTGGAGCCGAAAATGATGCCGAAACCTCAGCGGTTGCCGAGCCCAGTGACTGGGACGGTGACGGCAGCATGGA
TGCGGCGGTGGAAGACGGCGAATGGGGCAGCGACGGAATCGCGCCCGCCAGCGGCGCGCATGACGGTGACGACACCGAGG
TCAGCGAGCTGGCGCGCAGCCACGAATCCCTGACCGATTTCCTGCATCGCCAGGCATTGTCACTGCGCCTGTCCGATGTC
GACATGGCGGCGCTGCGCTTTCTCATCGAGTCGCTGAACGACGATGGCTACCTGGACGAGCCGCTGGAATCCCTGGCGGC
CAGCCTGGCGGGGGCCGACGACCCCGAGCAGCTCGAAGAGCTGGTGCACCGCTTCACTGTGGCCCTGCGCCTGCTGCAAA
ACCTGGAGCCGACCGGCGTGGGCGCGCGCGACCTGGCCGAATGCCTGACGCTACAGCTGCACGCCATGAACCGCGACGAA
GAGGGCGACCCGGCGGTGATTCAGACCGCGCTCGCCATCTGCAGCCAGCCGCTGGATCTGCTGGCGCGGCGCGACCTGCG
CCGCCTGGCCCAGGCCACAGGCAGCAGCGACGACGACACGCGTGCCGCCATGGCGCTGATCGCGCGCCTGGAGCCGCGCC
CGGGCCGGCGCTTTGCCGACGTGGAGCGCAACGTCATCGCGCCCGATGTCATCGTGAAAAAAACCGGACGCGGCGCGCAG
CAGGACTTCAGCGTGCAGCTCAACCCCGACGTCATGCCGCGCCTGCGCGTGCACGAGATCTATGCCAGCGCCCTGCGTGG
TGCGCGCGGCGGCGACGGGCACCAGGCGCTGCAGTCCCGGCTGCAGGAGGCACGCTGGTTCATCAAGAACATACAGCAGC
GATTTGACACTATCTTGCGTGTATCGCGCGCCATCGTCGAGCGGCAAAAGAGCTTTTTCGTGCATGGCGAGCTGGCCATG
CGGCCGCTGGTGCTGCGCGAGATCGCCGACGAGCTGGGCCTGCACGAGTCCACCATCAGCCGCGTGACCAGTGCCAAGTA
CATGGCCACGCCGCAGGGTACGTTCGAGTTGAAGTACTTCTTTGGCTCCGGCCTGGGCACCGAGACCGGCGGCAACGCGT
CGAGCACCGCGGTGCGCGCGCTGATCAAGCAGTTCGTCGCCGCCGAGGATGTGAAGAAACCGTTGTCGGACAACCAGATC
GCGGAGATGCTCAAGGAGCAGGGCATAGAGTGCGCGCGTCGCACCGTGGCCAAGTACCGCGAGGCGCTGAAGATCGCGCC
GGCCAATCTGCGCAAGGCGCTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-341)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(356-512)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.612

100

0.549