Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R1T29_RS01730 Genome accession   NZ_CP136903
Coordinates   359350..360366 (+) Length   338 a.a.
NCBI ID   WP_140067326.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain X1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354350..365366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T29_RS01715 (R1T29_01715) - 354774..355502 (-) 729 WP_015297397.1 redoxin family protein -
  R1T29_RS01720 (R1T29_01720) oxyR 355673..356581 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  R1T29_RS01725 (R1T29_01725) - 356637..359207 (-) 2571 WP_140265672.1 penicillin-binding protein 1A -
  R1T29_RS01730 (R1T29_01730) pilM 359350..360366 (+) 1017 WP_140067326.1 type IV pilus biogenesis protein PilM Machinery gene
  R1T29_RS01735 (R1T29_01735) pilN 360350..360928 (+) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  R1T29_RS01740 (R1T29_01740) pilO 360921..361514 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  R1T29_RS01745 (R1T29_01745) pilP 361504..362019 (+) 516 WP_025554074.1 pilus assembly protein PilP Machinery gene
  R1T29_RS01750 (R1T29_01750) pilQ 362051..363799 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  R1T29_RS01755 (R1T29_01755) aroK 363988..364506 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8322

>NTDB_id=893276 R1T29_RS01730 WP_140067326.1 359350..360366(+) (pilM) [Vibrio parahaemolyticus strain X1]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=893276 R1T29_RS01730 WP_140067326.1 359350..360366(+) (pilM) [Vibrio parahaemolyticus strain X1]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCAGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482