Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABK13_RS16425 Genome accession   NZ_AP027697
Coordinates   3384843..3385520 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain PSS-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3379843..3390520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABK13_RS16410 (VEE19_31710) chiA 3381210..3383903 (+) 2694 WP_000773174.1 bifunctional chitinase/lysozyme -
  AABK13_RS16415 (VEE19_31720) bfd 3384072..3384266 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABK13_RS16420 (VEE19_31730) bfr 3384338..3384814 (+) 477 WP_000675504.1 bacterioferritin -
  AABK13_RS16425 (VEE19_31740) pilD 3384843..3385520 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  AABK13_RS16430 (VEE19_31750) gspM 3385520..3385981 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  AABK13_RS16435 (VEE19_31760) gspL 3385978..3387141 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  AABK13_RS16440 (VEE19_31770) gspK 3387156..3388139 (-) 984 Protein_3208 type II secretion system minor pseudopilin GspK -
  AABK13_RS16445 (VEE19_31780) gspJ 3388132..3388719 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  AABK13_RS16450 (VEE19_31790) gspI 3388712..3389089 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABK13_RS16455 (VEE19_31800) gspH 3389086..3389595 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  AABK13_RS16460 (VEE19_31810) gspG 3389603..3390040 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=89294 AABK13_RS16425 WP_000178161.1 3384843..3385520(-) (pilD) [Escherichia coli strain PSS-12]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=89294 AABK13_RS16425 WP_000178161.1 3384843..3385520(-) (pilD) [Escherichia coli strain PSS-12]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378