Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WN937_RS14435 Genome accession   NZ_CP152363
Coordinates   3031113..3032579 (+) Length   488 a.a.
NCBI ID   WP_025508884.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain vp-HL-201910     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3026113..3037579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WN937_RS14410 (WN937_14410) - 3027233..3028198 (+) 966 WP_005455122.1 calcium/sodium antiporter -
  WN937_RS14415 (WN937_14415) kdsD 3028235..3029206 (+) 972 WP_025588216.1 arabinose-5-phosphate isomerase KdsD -
  WN937_RS14420 (WN937_14420) lptC 3029297..3029860 (+) 564 WP_005467805.1 LPS export ABC transporter periplasmic protein LptC -
  WN937_RS14425 (WN937_14425) lptA 3029841..3030335 (+) 495 WP_005467803.1 lipopolysaccharide transport periplasmic protein LptA -
  WN937_RS14430 (WN937_14430) lptB 3030337..3031062 (+) 726 WP_005467801.1 LPS export ABC transporter ATP-binding protein -
  WN937_RS14435 (WN937_14435) rpoN/rpoN1 3031113..3032579 (+) 1467 WP_025508884.1 RNA polymerase factor sigma-54 Machinery gene
  WN937_RS14440 (WN937_14440) hpf 3032604..3032891 (+) 288 WP_005467451.1 ribosome hibernation promoting factor -
  WN937_RS14445 (WN937_14445) ptsN 3032894..3033340 (+) 447 WP_005467453.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WN937_RS14450 (WN937_14450) rapZ 3033355..3034218 (+) 864 WP_005468663.1 RNase adapter RapZ -
  WN937_RS14455 (WN937_14455) - 3034221..3034496 (+) 276 WP_005468661.1 HPr family phosphocarrier protein -
  WN937_RS14460 (WN937_14460) mgtE 3034716..3036071 (+) 1356 WP_005468659.1 magnesium transporter -
  WN937_RS14465 (WN937_14465) pmbA 3036148..3037491 (-) 1344 WP_005459755.1 metalloprotease PmbA -

Sequence

Protein


Download         Length: 488 a.a.        Molecular weight: 54837.68 Da        Isoelectric Point: 4.3618

>NTDB_id=892261 WN937_RS14435 WP_025508884.1 3031113..3032579(+) (rpoN/rpoN1) [Vibrio parahaemolyticus strain vp-HL-201910]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLEVEEGHEEPQANGEDKSALETADNSANEPTEIE
VPDSSDVIEKSEISSELEIDTTWDDVYSANTGSTGLALDDDMPVYQGETTESLHDYLMWQLDLTPFSETDRTIAIAIIDA
IDDYGYLTLSPEEIHESFNNEDIELDEVEAVRKRIQQFDPLGVASRNLQECLLLQLATFPEDTPWLAEAKMILADHIDHL
GNRDYKLVIKETKLKEADLREVLKLIQQLDPRPGSRITPDDTEYVIPDVSVFKDHGKWTVSINPDSIPKLKVNQQYAQLG
KGNSADSQYIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQQDFFEYGEEAMKPMVLNDVALAVDMHESTISRVTTQKF
MHTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENTAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAP
SSQRKRLL

Nucleotide


Download         Length: 1467 bp        

>NTDB_id=892261 WN937_RS14435 WP_025508884.1 3031113..3032579(+) (rpoN/rpoN1) [Vibrio parahaemolyticus strain vp-HL-201910]
ATGAAACCTTCATTACAACTCAAGCTAGGTCAACAGTTAGCCATGACGCCACAGTTGCAACAAGCGATTCGCTTGCTGCA
GTTGTCGACTCTCGATCTTCAACAAGAAATCCAAGAAGCACTGGACTCAAACCCACTATTGGAAGTTGAAGAAGGCCACG
AAGAACCTCAAGCAAATGGTGAAGACAAATCAGCACTCGAGACTGCTGACAACAGTGCCAACGAGCCAACAGAAATCGAA
GTTCCTGATAGTTCTGACGTCATCGAAAAGTCCGAAATCAGCTCAGAGCTTGAAATCGACACAACTTGGGATGACGTCTA
CAGTGCAAATACGGGCAGTACCGGTTTAGCGTTAGACGATGATATGCCGGTCTATCAAGGCGAAACCACAGAATCGCTAC
ACGATTACCTGATGTGGCAACTCGACTTAACGCCTTTCAGTGAAACCGACCGTACGATTGCCATTGCCATCATTGATGCG
ATTGACGATTACGGCTATCTGACCCTATCTCCTGAAGAGATTCACGAAAGCTTCAACAACGAAGATATTGAGTTGGATGA
AGTCGAAGCCGTGCGCAAACGCATCCAACAGTTTGACCCGCTTGGTGTGGCATCGCGGAACCTGCAAGAATGCTTACTCC
TGCAACTTGCAACCTTCCCAGAAGACACGCCTTGGCTTGCAGAAGCCAAAATGATTCTTGCCGATCACATCGATCACCTT
GGTAATCGCGATTACAAACTGGTCATCAAAGAAACTAAGCTAAAAGAAGCGGATCTACGCGAAGTATTAAAACTGATCCA
ACAGTTAGATCCTCGTCCGGGCAGCCGCATTACCCCAGACGACACAGAATACGTCATTCCGGATGTATCGGTATTTAAAG
ATCATGGAAAATGGACCGTTTCCATCAACCCTGACAGCATTCCAAAGTTAAAAGTTAACCAACAATACGCTCAATTAGGC
AAAGGCAACAGTGCGGATAGCCAGTACATTCGCAGCAATTTGCAAGAGGCAAAATGGTTAATTAAGAGCTTAGAGAGCAG
AAACGAGACGCTACTCAAAGTTGCAAGATGTATTGTTGAACATCAACAAGATTTCTTCGAGTATGGTGAAGAAGCCATGA
AGCCAATGGTACTCAATGATGTAGCCTTGGCGGTGGACATGCATGAATCCACAATATCTCGTGTTACGACACAAAAGTTC
ATGCATACGCCACGTGGCATTTTTGAATTGAAGTACTTCTTCTCCAGCCACGTCAGTACAGACAATGGCGGAGAATGTTC
GTCAACAGCAATCCGTGCACTCATTAAGAAACTGGTCGCGGCAGAGAATACTGCTAAACCACTTAGTGACAGTAAGATTG
CTGCTCTTCTGGCTGACCAAGGGATTCAGGTCGCAAGACGTACGATAGCTAAATATCGAGAATCCTTGGGTATTGCCCCT
TCAAGTCAGCGTAAACGCCTACTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(328-486)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-316)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.444

100

0.459