Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAC934_RS13230 Genome accession   NZ_CP152362
Coordinates   2571638..2572048 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain LP     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2566638..2577048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAC934_RS13200 (AAC934_13200) - 2566725..2566865 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  AAC934_RS13205 (AAC934_13205) yqeF 2567171..2567902 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  AAC934_RS13210 (AAC934_13210) cwlH 2568154..2568906 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  AAC934_RS13215 (AAC934_13215) yqeD 2569093..2569719 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  AAC934_RS13220 (AAC934_13220) gnd 2569738..2570631 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  AAC934_RS13225 (AAC934_13225) yqeB 2570883..2571605 (+) 723 WP_342623042.1 DUF308 domain-containing protein -
  AAC934_RS13230 (AAC934_13230) nucA/comI 2571638..2572048 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  AAC934_RS13235 (AAC934_13235) - 2572244..2572588 (+) 345 Protein_2570 sigma-70 family RNA polymerase sigma factor -
  AAC934_RS13240 (AAC934_13240) - 2572734..2573204 (+) 471 WP_069322767.1 MarR family transcriptional regulator -
  AAC934_RS13245 (AAC934_13245) - 2573354..2574766 (+) 1413 WP_342623047.1 MDR family MFS transporter -
  AAC934_RS13250 (AAC934_13250) spoIVCA 2574953..2576413 (-) 1461 WP_342625727.1 site-specific DNA recombinase SpoIVCA -
  AAC934_RS13255 (AAC934_13255) - 2576371..2576549 (-) 179 Protein_2574 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=892144 AAC934_RS13230 WP_009967785.1 2571638..2572048(-) (nucA/comI) [Bacillus subtilis strain LP]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=892144 AAC934_RS13230 WP_009967785.1 2571638..2572048(-) (nucA/comI) [Bacillus subtilis strain LP]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCAGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTTCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529