Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AABK10_RS17065 Genome accession   NZ_AP027694
Coordinates   3455601..3456278 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain PSS-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3450601..3461278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABK10_RS17050 (VEE21_32340) chiA 3451968..3454661 (+) 2694 WP_222698513.1 bifunctional chitinase/lysozyme -
  AABK10_RS17055 (VEE21_32350) bfd 3454830..3455024 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  AABK10_RS17060 (VEE21_32360) bfr 3455096..3455572 (+) 477 WP_000675504.1 bacterioferritin -
  AABK10_RS17065 (VEE21_32370) pilD 3455601..3456278 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  AABK10_RS17070 (VEE21_32380) gspM 3456278..3456739 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  AABK10_RS17075 (VEE21_32390) gspL 3456736..3457899 (-) 1164 WP_096955985.1 type II secretion system protein GspL -
  AABK10_RS17080 (VEE21_32400) gspK 3457914..3458897 (-) 984 WP_114476607.1 type II secretion system minor pseudopilin GspK -
  AABK10_RS17085 (VEE21_32410) gspJ 3458890..3459477 (-) 588 WP_000610633.1 type II secretion system minor pseudopilin GspJ -
  AABK10_RS17090 (VEE21_32420) gspI 3459470..3459847 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  AABK10_RS17095 (VEE21_32430) gspH 3459844..3460353 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  AABK10_RS17100 (VEE21_32440) gspG 3460361..3460798 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=89213 AABK10_RS17065 WP_000178161.1 3455601..3456278(-) (pilD) [Escherichia coli strain PSS-11]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=89213 AABK10_RS17065 WP_000178161.1 3455601..3456278(-) (pilD) [Escherichia coli strain PSS-11]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378