Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   RAS18_RS05670 Genome accession   NZ_CP136635
Coordinates   1140072..1143776 (+) Length   1234 a.a.
NCBI ID   WP_406621635.1    Uniprot ID   -
Organism   Bacillus atrophaeus subsp. globigii strain Hab-5     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1135072..1148776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAS18_RS05655 - 1135530..1135855 (-) 326 Protein_1057 four-helix bundle copper-binding protein -
  RAS18_RS05660 - 1135942..1136379 (-) 438 WP_150223671.1 ferritin family protein -
  RAS18_RS05665 addB 1136585..1140085 (+) 3501 WP_406621634.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RAS18_RS05670 addA 1140072..1143776 (+) 3705 WP_406621635.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RAS18_RS05675 sbcD 1143847..1145025 (+) 1179 WP_406621636.1 exonuclease subunit SbcD -
  RAS18_RS05680 sbcC 1145022..1148414 (+) 3393 WP_406621637.1 exonuclease subunit SbcC -
  RAS18_RS05685 - 1148431..1148733 (+) 303 WP_106045311.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141502.13 Da        Isoelectric Point: 5.5155

>NTDB_id=892014 RAS18_RS05670 WP_406621635.1 1140072..1143776(+) (addA) [Bacillus atrophaeus subsp. globigii strain Hab-5]
MQIPKPADSTWTDDQWNAIVSSGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLRKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPKTWLEGFVHLYDASEKSTIEELPFYPYMKEDIAMVLSAVKEKLQRA
LELTKVPGGPAPRADNFLDDLAQTEDLIAHSADFTELYKRVPAVSFKRAKAVKGDEFDQTLTEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLSDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAVNESGERKPSEAAAYYQEQ
FQEVLVDEYQDTNLVQEAILQLVTSGPEASGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTETGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKVGEVDYDEQAELKLGASYPPNDQTETELLLIDRAEEKEASEETEELETVQLEAKAIAKQIR
KLVSSPYKVYDGKSKTHSNIRYRDIVILLRSMPWAPQIMEELKAQGIPVYANLTSGYFEAVEVSVAISALKVIDNPYQDI
PLASVLRSPIVGADENELSLIRMHNKKAPFYEAMKDYLSGGDRNDELYQKLNRFFELLQKWRSFSKNHSVSELIWEVYRD
SKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRQELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQASASQPDWLLPDFERYQAKTYLDFIGPALIRHRDMGADALQGLASQADISEHPARFNIQMVSAQD
LLDDDLEERQEEKSGRLDAIRRGEPVSGSFEFEEQARQRLSWRYPFHDVTKIRTKQSVSEIKRKKEYEDEYSGRSLIRPA
DGNMLYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHAPSKEEAKRIVKRLYEKELLTEDQQAVIDLEEIVQFFHTEIGE
KLIGAKWINREVPFSMALSAQDAFLDTESKDEPILVQGIIDCLYETEEGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIELYTKAVEQITKTNVKGRALYFFDGGHVLNV

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=892014 RAS18_RS05670 WP_406621635.1 1140072..1143776(+) (addA) [Bacillus atrophaeus subsp. globigii strain Hab-5]
ATGCAAATTCCTAAACCGGCAGACAGCACCTGGACAGATGACCAGTGGAACGCCATTGTCTCATCCGGACAGGATATTCT
CGTTGCAGCGGCCGCTGGATCGGGAAAAACCGCTGTGCTTGTTGAGAGAATGATCCGCAAAATTACCGCGGAGGAAAATC
CGATAGATGTGGACCGTCTTTTGGTCGTGACATTTACGAACGCATCTGCGGCTGAAATGAAGCACCGGATCGCGGAGGCT
TTGGAAAAAGAGCTCGTGAAACGTCCCGGCTCTCTTCATATCAGGCGGCAGCTGTCTCTGTTGAACCGGGCCAGCATTTC
AACCCTTCATTCGTTCTGCTTGCAGGTGCTCAGAAAATATTATTATCTCATTGATCTTGATCCGGGCTTTCGAATTGCCG
ATCAGACAGAAGGCGAGCTGCTCGGGGACGAGGTCCTTGATGAACTGTTTGAAGACGAATACGCGAAGGGTGAAAAAGCG
TTTTTCGAACTGGTGGACCGGTATACGACAGACCGACACGACTTAGACCTTCAGCACCTCGTGAAACGGGTGTATGAGTT
TTCCCGTTCCCATCCCGATCCAAAAACGTGGCTGGAAGGTTTCGTTCATCTTTACGATGCCTCAGAAAAAAGCACGATTG
AAGAACTGCCGTTTTATCCATATATGAAAGAAGACATTGCGATGGTGCTGAGCGCTGTGAAAGAAAAGCTTCAGCGGGCG
CTTGAACTGACAAAAGTGCCGGGCGGCCCTGCGCCGCGCGCTGATAATTTTCTGGATGATCTTGCACAGACAGAGGATCT
GATCGCACATTCGGCGGATTTCACTGAATTATACAAGCGGGTTCCCGCAGTCTCCTTTAAGCGTGCCAAAGCGGTAAAAG
GCGATGAATTTGACCAGACGCTCACAGAAGAAGCGACGGACTTACGGAACGGCGCGAAAAAACTGCTGGAAAAACTAAAA
ACGGACTATTTCACAAGAAGCCCCGAGCAGCATCTGAAGAGCCTGTCGGATATGAAGCCTGTGATTGAAACGCTCGTCCA
GCTTGTCATCAGCTACGGAAAACGGTTTGAAGCCGCCAAACAAGAGAAATCAATTATTGATTTCTCTGACTTGGAGCATT
ACTGCTTGTCTATCCTGACAGCCGTGAATGAGTCGGGGGAGCGCAAGCCGAGTGAGGCGGCGGCTTACTATCAAGAGCAG
TTCCAAGAAGTGCTGGTTGACGAATATCAAGATACGAATCTTGTGCAGGAAGCCATACTGCAGCTTGTCACAAGCGGGCC
TGAAGCTTCAGGAAACTTGTTTATGGTAGGGGATGTCAAACAGTCCATCTATCGATTCAGGCTTGCCGAACCGCTTCTTT
TCCTTTCTAAATATAAACGCTTTACCGAAACGGGAGAGGGGACAGGCCGGAAAATTGATTTGAACAAAAACTTTCGAAGC
CGGGCGGATATTTTAGACAGCACGAACTTTCTGTTTAAGCAGCTCATGGGCGGCAAGGTCGGCGAGGTTGATTATGATGA
GCAGGCGGAATTGAAGCTAGGAGCCTCTTACCCTCCGAATGACCAGACTGAAACGGAGCTGTTGCTGATTGACAGGGCGG
AGGAAAAAGAGGCGAGTGAAGAAACAGAAGAGCTCGAAACCGTTCAGCTTGAAGCAAAAGCGATTGCAAAGCAGATTCGA
AAGCTAGTATCCTCTCCTTACAAAGTCTATGATGGGAAATCGAAAACACACAGCAATATCCGCTACCGCGATATCGTGAT
TTTACTGCGTTCCATGCCGTGGGCGCCGCAAATCATGGAAGAACTGAAAGCTCAAGGCATACCCGTCTATGCCAATTTGA
CATCAGGCTATTTTGAAGCGGTCGAGGTGTCTGTTGCGATTTCCGCTTTAAAAGTGATTGATAATCCGTACCAAGATATT
CCTCTCGCTTCCGTGCTGCGCTCGCCGATTGTCGGCGCGGATGAAAATGAGCTTTCACTGATTCGGATGCATAATAAAAA
AGCGCCTTTCTATGAAGCGATGAAGGACTATCTTTCAGGCGGAGACCGAAATGACGAGCTATATCAAAAGCTGAACAGGT
TTTTTGAGCTTTTGCAAAAATGGCGCTCTTTTTCGAAAAACCATTCAGTTTCCGAGCTGATATGGGAAGTTTATCGTGAC
AGCAAATATATGGACTATGTCGGCGGTATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGTTCTATATGACCGGGC
CCGCCAGTATGAATCGACGGCATTTCGCGGTTTATTTCGTTTCTTGCGGTTTATAGAACGTATGCAGGAACGGGGCGATG
ACCTCGGGACAGCGAGAGCGCTCAGCGAGCAGGAGGACGTGGTCCGCCTGATGACGATTCACAGCAGCAAGGGGCTTGAA
TTCCCTGTCGTATTCGCTGCGGGACTTGGACGGAATTTTAATATGATGGATTTGAATAAATCATATCTGCTGGACAAAGA
GCTTGGTTTCGGCACAAAGTTTATTCACCCCGAATGGCGCATCAGCTATCCGACACTTCCACTCATCGCCATGAAGAAAA
AAATGCGCCAGGAGCTGCTGTCAGAAGAGCTGCGTGTGCTATACGTGGCTTTAACAAGAGCGAAGGAAAAGCTGTTTCTT
GTCGGTTCATGCAAGAATCGTGAGAAGCAGCTGGCGAAATGGCAGGCTTCAGCGTCTCAGCCAGACTGGCTTCTCCCGGA
TTTTGAGCGGTATCAGGCGAAAACGTATTTGGATTTCATCGGTCCCGCATTGATTCGTCACAGAGACATGGGGGCAGATG
CTTTACAAGGGCTTGCGAGTCAAGCTGACATATCAGAACACCCCGCCCGGTTTAACATCCAAATGGTCAGCGCCCAAGAT
TTATTGGATGATGATCTGGAAGAAAGACAAGAGGAAAAGAGCGGCCGTCTTGACGCCATCCGCCGCGGTGAGCCTGTGTC
CGGTTCGTTTGAATTCGAAGAACAAGCGCGGCAACGCCTGTCATGGCGATACCCTTTTCACGATGTGACAAAAATTCGCA
CGAAGCAGTCTGTTTCCGAGATCAAGAGAAAAAAAGAATACGAGGACGAATACAGCGGCCGTTCTCTCATCAGACCGGCT
GACGGAAACATGTTGTACAGGCGCCCTGCGTTTATGATGAAAAAAGGGCTGACTGCGGCTGAAAAAGGAACGGCGATGCA
TACCGTTATGCAGCATATCCCGCTGACACATGCGCCGTCAAAAGAGGAAGCGAAACGTATTGTCAAACGTCTTTATGAAA
AAGAGCTGCTCACGGAAGACCAGCAAGCCGTTATTGACCTAGAGGAAATCGTGCAATTTTTCCATACTGAAATCGGCGAA
AAGCTGATCGGCGCAAAATGGATTAACCGCGAAGTGCCGTTTAGCATGGCGCTTTCAGCACAAGATGCGTTTCTGGACAC
TGAATCTAAAGATGAGCCCATATTGGTGCAAGGAATTATTGACTGTCTGTATGAGACCGAGGAAGGGCTTTACTTGCTGG
ATTACAAAACGGACCGAATCGAAGGGAAATTCCAGCACGGATTTGAGGGGGCGGCTCCTATTCTTAAAAAACGATACGAA
ACTCAAATCGAGCTATACACAAAAGCTGTAGAACAAATAACGAAAACCAATGTGAAGGGACGTGCACTCTACTTCTTCGA
TGGAGGACACGTTTTGAACGTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

86.467

100

0.865