Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RZV14_RS18025 Genome accession   NZ_CP136576
Coordinates   4230540..4231673 (+) Length   377 a.a.
NCBI ID   WP_039403605.1    Uniprot ID   A0AB34PE08
Organism   Xanthomonas cannabis strain DAR82756     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4225540..4236673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV14_RS18000 (RZV14_17950) - 4225705..4226892 (-) 1188 WP_355585219.1 cystathionine gamma-synthase -
  RZV14_RS18005 (RZV14_17955) - 4226995..4228365 (-) 1371 WP_039403582.1 pyridoxal-phosphate dependent enzyme -
  RZV14_RS18010 (RZV14_17960) - 4228597..4228815 (-) 219 WP_005991804.1 YdcH family protein -
  RZV14_RS18015 (RZV14_17965) - 4229170..4230060 (-) 891 WP_166744244.1 hypothetical protein -
  RZV14_RS18020 (RZV14_17970) - 4230057..4230440 (-) 384 WP_166744243.1 DUF4398 domain-containing protein -
  RZV14_RS18025 (RZV14_17975) pilU 4230540..4231673 (+) 1134 WP_039403605.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZV14_RS18030 (RZV14_17980) maiA 4232138..4232800 (-) 663 WP_228334464.1 maleylacetoacetate isomerase -
  RZV14_RS18035 (RZV14_17985) - 4232844..4233833 (-) 990 WP_228334465.1 fumarylacetoacetate hydrolase family protein -
  RZV14_RS18040 (RZV14_17990) - 4234212..4235594 (+) 1383 WP_039403615.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41764.15 Da        Isoelectric Point: 6.1401

>NTDB_id=891643 RZV14_RS18025 WP_039403605.1 4230540..4231673(+) (pilU) [Xanthomonas cannabis strain DAR82756]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEAAASPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=891643 RZV14_RS18025 WP_039403605.1 4230540..4231673(+) (pilU) [Xanthomonas cannabis strain DAR82756]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGAATGGTCAAGAAAATCGCCTATTCCT
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCCGACGCGGGCCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTAGTGGGGTCCACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACACACGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCTTGATCGGCGAGATCCTGGACGCCGAGACCATGGAGGCGG
CCATCGCGTTCGCCGAAACCGGCCACCTGTGCTTGGCCACGCTGCACTCCAACAATGCTGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTGTCGCTACG
CCTGGTCAAGGGCGTGGACGGACGCCGCCGGCCGGCGACCGAGGTACTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GGCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAAGAGGCGCTGCGCGCCGCCGATTCACGCGATGGCCTGGCGCTGAA
ATTCCGCCTGTCGGAAGGTGGCTCGGGCGAGCATGACCCTTACGCGGATTACGAAGCGGCGGCTTCGCCGAAGATCAGTC
ACGGGTTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

48.889

95.491

0.467
  pilU Acinetobacter baylyi ADP1

49.153

93.899

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.448
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361