Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAGT87_RS15085 Genome accession   NZ_CP152071
Coordinates   2247989..2249452 (-) Length   487 a.a.
NCBI ID   WP_000804830.1    Uniprot ID   Q9KP49
Organism   Vibrio cholerae strain Sudan_2019_18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2242989..2254452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT87_RS15055 (AAGT87_15060) pmbA 2243131..2244474 (+) 1344 WP_353495233.1 metalloprotease PmbA -
  AAGT87_RS15060 (AAGT87_15065) mgtE 2244546..2245907 (-) 1362 WP_353495234.1 magnesium transporter -
  AAGT87_RS15065 (AAGT87_15070) - 2246068..2246346 (-) 279 WP_001135899.1 HPr family phosphocarrier protein -
  AAGT87_RS15070 (AAGT87_15075) rapZ 2246355..2247215 (-) 861 WP_353495235.1 RNase adapter RapZ -
  AAGT87_RS15075 (AAGT87_15080) ptsN 2247228..2247674 (-) 447 WP_001177163.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAGT87_RS15080 (AAGT87_15085) hpf 2247677..2247964 (-) 288 WP_001166643.1 ribosome hibernation promoting factor -
  AAGT87_RS15085 (AAGT87_15090) rpoN/rpoN1 2247989..2249452 (-) 1464 WP_000804830.1 RNA polymerase factor sigma-54 Machinery gene
  AAGT87_RS15090 (AAGT87_15095) lptB 2249508..2250233 (-) 726 WP_000996178.1 LPS export ABC transporter ATP-binding protein -
  AAGT87_RS15095 (AAGT87_15100) lptA 2250236..2250730 (-) 495 WP_000830444.1 lipopolysaccharide transport periplasmic protein LptA -
  AAGT87_RS15100 (AAGT87_15105) lptC 2250711..2251274 (-) 564 WP_000059348.1 LPS export ABC transporter periplasmic protein LptC -
  AAGT87_RS15105 (AAGT87_15110) kdsC 2251271..2251828 (-) 558 WP_174499304.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AAGT87_RS15110 (AAGT87_15115) kdsD 2251828..2252802 (-) 975 WP_001120614.1 arabinose-5-phosphate isomerase KdsD -
  AAGT87_RS15115 (AAGT87_15120) - 2252858..2253820 (-) 963 WP_000904330.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 55438.47 Da        Isoelectric Point: 4.2824

>NTDB_id=891470 AAGT87_RS15085 WP_000804830.1 2247989..2249452(-) (rpoN/rpoN1) [Vibrio cholerae strain Sudan_2019_18]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEENQDEAPSLDSVRMTEESPREPEELYEPEP
QDSSDLIEKSEISAELEMDTTWDEVYSANTGSTGLALDDDAPIYQGETTQTLQDYLHWQLDLTPFSDVDRTIAVALIDAI
DDYGYLTVSLEEIQESLRSDDIELDEIEAVRKRIQQFDPFGVASLNLQDCLLLQLTTYPCDTPWLEEARLLLSQYIDDLG
NRDYKTILKETKLKEEDLREILQLIQQLDPRPGSRIAQDHAEYVIPDVSVYKEQGRWLVTINPDSVPKLKINQQYADLMR
GNNAESNYIRTNLQEAKWLIKSLESRNETLLKVAKCIVEHQHDFFEYGEEAMKPMVLNDVAMAVEMHESTISRVTTQKYM
HTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENPAKPLSDSKIATLLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=891470 AAGT87_RS15085 WP_000804830.1 2247989..2249452(-) (rpoN/rpoN1) [Vibrio cholerae strain Sudan_2019_18]
ATGAAACCGTCATTACAGCTCAAGCTAGGTCAACAGTTAGCCATGACTCCGCAGCTCCAGCAGGCGATTCGTCTGTTGCA
GCTTTCGACGCTGGATCTGCAGCAAGAAATTCAGGAAGCATTGGAATCTAACCCCTTGTTAGAGGTGGAAGAGAACCAAG
ACGAAGCGCCATCGCTTGATAGCGTACGTATGACGGAAGAAAGCCCGCGCGAGCCAGAAGAACTCTACGAACCTGAACCG
CAGGATAGCTCGGATCTGATTGAAAAATCGGAAATCAGCGCTGAATTAGAGATGGATACCACTTGGGATGAAGTCTACAG
CGCCAATACTGGCAGTACAGGACTGGCACTCGATGATGATGCCCCTATCTACCAAGGCGAAACCACCCAAACCCTGCAAG
ATTATCTTCACTGGCAACTCGACTTAACTCCCTTTAGCGATGTGGATCGCACTATAGCCGTCGCACTTATCGATGCTATC
GACGATTACGGTTATTTAACCGTCTCACTAGAGGAGATCCAAGAGAGCCTGCGCAGTGATGACATTGAGTTGGATGAGAT
TGAAGCGGTACGTAAACGCATTCAGCAGTTTGACCCCTTTGGTGTGGCATCGCTTAATCTGCAAGACTGCTTGTTGCTGC
AACTCACCACCTATCCCTGTGATACTCCTTGGCTGGAAGAAGCGCGTTTACTGCTCTCACAGTACATCGATGATTTAGGG
AATCGCGATTACAAAACCATTCTGAAAGAGACTAAGCTCAAAGAAGAGGACTTGCGCGAGATCCTGCAACTCATCCAACA
GCTCGATCCTCGCCCCGGCAGTCGAATTGCCCAAGATCACGCTGAATACGTCATACCTGACGTGTCAGTCTATAAAGAAC
AAGGTCGATGGCTGGTCACCATCAACCCAGATAGTGTGCCTAAACTGAAGATCAATCAGCAGTATGCCGACCTGATGCGC
GGTAATAATGCGGAGAGCAACTACATCCGTACCAATTTGCAAGAGGCAAAATGGCTGATCAAAAGTTTAGAAAGTAGAAA
CGAGACACTGCTCAAAGTTGCCAAGTGCATAGTTGAACACCAACACGATTTCTTCGAGTATGGAGAAGAGGCGATGAAGC
CGATGGTGCTCAACGATGTGGCGATGGCGGTGGAAATGCATGAATCGACAATTTCGCGTGTCACCACGCAGAAATACATG
CATACCCCGCGCGGCATTTTTGAGTTGAAGTACTTTTTCTCCAGCCACGTCAGTACTGACAATGGCGGAGAGTGCTCGTC
CACTGCGATCAGGGCGCTGATCAAAAAACTGGTGGCGGCAGAGAATCCTGCCAAACCACTCAGTGACAGCAAGATCGCAA
CCCTACTCGCTGACCAAGGCATCCAAGTCGCGCGGCGAACCATCGCCAAATACCGTGAATCACTTGGCATCGCCCCATCA
AGTCAGCGCAAACGCCTGCTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KP49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.533

100

0.46