Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AAGT87_RS14385 Genome accession   NZ_CP152071
Coordinates   2113809..2116013 (+) Length   734 a.a.
NCBI ID   WP_001247858.1    Uniprot ID   Q9KNQ3
Organism   Vibrio cholerae strain Sudan_2019_18     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2108809..2121013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT87_RS14365 (AAGT87_14370) - 2109889..2111055 (-) 1167 WP_341264776.1 O-succinylhomoserine (thiol)-lyase -
  AAGT87_RS14370 (AAGT87_14375) metJ 2111323..2111640 (+) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  AAGT87_RS14375 (AAGT87_14380) - 2111751..2113028 (-) 1278 WP_001278588.1 malic enzyme-like NAD(P)-binding protein -
  AAGT87_RS14380 (AAGT87_14385) rpmE 2113336..2113554 (-) 219 WP_000643446.1 50S ribosomal protein L31 -
  AAGT87_RS14385 (AAGT87_14390) priA 2113809..2116013 (+) 2205 WP_001247858.1 primosomal protein N' Machinery gene
  AAGT87_RS14390 (AAGT87_14395) cytR 2116319..2117326 (+) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  AAGT87_RS14395 (AAGT87_14400) - 2117498..2118049 (+) 552 WP_001884022.1 SPOR domain-containing protein -
  AAGT87_RS14400 (AAGT87_14405) hslV 2118186..2118743 (+) 558 WP_353495221.1 ATP-dependent protease subunit HslV -
  AAGT87_RS14405 (AAGT87_14410) hslU 2118809..2120140 (+) 1332 WP_341261145.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82080.38 Da        Isoelectric Point: 8.4853

>NTDB_id=891462 AAGT87_RS14385 WP_001247858.1 2113809..2116013(+) (priA) [Vibrio cholerae strain Sudan_2019_18]
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPRLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=891462 AAGT87_RS14385 WP_001247858.1 2113809..2116013(+) (priA) [Vibrio cholerae strain Sudan_2019_18]
ATGCGACCCTCCATTGCCCGAGTTTGCCTACCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTGCAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATCCGTGCAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGCCAGTTTTATCATTATCCGCTCGGTGAAACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGCAAAGCGGCAGAGCTGACTAGCCATAAAGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAACTGATGCAAGGGGTGA
AGCGTGGCGCGGTAAAACAAGCGCAAGTTCTCCATTTGTTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGCACGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATTGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAGGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAGCAAGGTTTCGGCTGCTTTTTATTAGAAGGGGTAACGGGCTCAGGCAAAACAGAGGTGTATCTTAACCTCATCACA
CCGGTATTGGCGCGTGGTGAACAAGCCTTGGTGTTGGTGCCGGAAATTGGTTTAACCCCACAAACCATCAACCGGTTTCG
CCAACGTTTTAATGTCCCCGTCGAAGTCATGCACTCGGCGCTCAACGATACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACGCGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTACAAGCAGCAAGATAGCCTGCGTTATCACGCCCGCGATGTGGCTGTGATGCGCGCGCA
TCTGGAGAATATTCCGATTGTGCTTGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCAGTGGTAAATACC
ATCACTTGCAACTGACGCAGCGTGCTGGTAATGCACTACCCACACGTAACCACGCGTTGGATGTGAAAGGGCTCTATCTG
GAAAGTGGTCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGCCATGAATGCGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAGATGCGCTGCCACCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTTACCTTGGTCGCGTTGTTGGATGTC
GATGGCTCATTGTACAGCAGTGATTTTCGCGCTTCTGAGCGTTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGCGAAGTGGTGCTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGTAAGCTGGCGCAGTTGCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCTGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTCGGGCCAACGCCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAGCTTTTAC
TACAAACGCCGAATCGCACCCTGATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGGCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(204-364)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

Helicase conserved C-terminal domain (Helicase_C)

(515-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KNQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.572

100

0.439