Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAFX34_RS21530 Genome accession   NZ_CP151937
Coordinates   3043826..3045289 (+) Length   487 a.a.
NCBI ID   WP_045623868.1    Uniprot ID   -
Organism   Vibrio vulnificus strain TBV029     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3038826..3050289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX34_RS21500 (AAFX34_22055) - 3039497..3040462 (+) 966 WP_013572480.1 calcium/sodium antiporter -
  AAFX34_RS21505 (AAFX34_22060) kdsD 3040484..3041455 (+) 972 WP_045623869.1 arabinose-5-phosphate isomerase KdsD -
  AAFX34_RS21510 (AAFX34_22065) kdsC 3041455..3042012 (+) 558 WP_013572482.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AAFX34_RS21515 (AAFX34_22070) lptC 3042009..3042572 (+) 564 WP_011078767.1 LPS export ABC transporter periplasmic protein LptC -
  AAFX34_RS21520 (AAFX34_22075) lptA 3042553..3043047 (+) 495 WP_011078768.1 lipopolysaccharide transport periplasmic protein LptA -
  AAFX34_RS21525 (AAFX34_22080) lptB 3043047..3043772 (+) 726 WP_011078769.1 LPS export ABC transporter ATP-binding protein -
  AAFX34_RS21530 (AAFX34_22085) rpoN/rpoN1 3043826..3045289 (+) 1464 WP_045623868.1 RNA polymerase factor sigma-54 Machinery gene
  AAFX34_RS21535 (AAFX34_22090) hpf 3045313..3045600 (+) 288 WP_011078771.1 ribosome hibernation promoting factor -
  AAFX34_RS21540 (AAFX34_22095) ptsN 3045603..3046049 (+) 447 WP_011078772.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAFX34_RS21545 (AAFX34_22100) rapZ 3046062..3046925 (+) 864 WP_011078773.1 RNase adapter RapZ -
  AAFX34_RS21550 (AAFX34_22105) - 3046928..3047203 (+) 276 WP_011078774.1 HPr family phosphocarrier protein -
  AAFX34_RS21555 (AAFX34_22110) mgtE 3047418..3048773 (+) 1356 WP_011078775.1 magnesium transporter -
  AAFX34_RS21560 (AAFX34_22115) pmbA 3048875..3050218 (-) 1344 WP_011078776.1 metalloprotease PmbA -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 54630.61 Da        Isoelectric Point: 4.3541

>NTDB_id=891395 AAFX34_RS21530 WP_045623868.1 3043826..3045289(+) (rpoN/rpoN1) [Vibrio vulnificus strain TBV029]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEEHQEEAQLNGEEKAINEGSDSDDFSVEIDI
PDSSDVIEKSEISSELEIDTTWDDVYSANSGNTGIALDDDMPVYQGETTETLHDYLQWQLDLTPFSDTDRMIAFAIIDAV
DDTGYLTISPEEILESLNSVDIELDEVEAVRKRIQQFDPLGVASQNLQECLLLQLATFPIETPWLAEAKMVLSHHIDALG
NRDYKLIVKEAKLKEEDLRETLKLIQQLDPRPGSKINAIEPEYVIPDVSVFKENGKWSVAINPDSIPKLKVNQQYAQLGK
GSSADSQYIRSNLQEAKWLIKSLESRNETLLKVARCIVEHQHDFFEYGEEAMKPMVLNDVATAVEMHESTISRVTTQKFM
HTPRGIFELKYFFSSHVSTDSGGECSSTAIRALIKKLVTAENSAKPLSDSKIAALLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=891395 AAFX34_RS21530 WP_045623868.1 3043826..3045289(+) (rpoN/rpoN1) [Vibrio vulnificus strain TBV029]
ATGAAACCTTCATTACAACTCAAGCTAGGTCAACAGTTAGCCATGACGCCTCAGCTCCAGCAGGCCATACGTTTGCTGCA
GCTGTCCACCTTGGATTTACAACAAGAAATCCAAGAAGCGCTGGAATCAAATCCCTTATTGGAAGTGGAAGAGCATCAAG
AAGAAGCGCAACTGAATGGCGAAGAGAAAGCGATCAATGAAGGTTCCGATTCTGATGATTTTTCTGTTGAGATCGACATT
CCCGACAGCTCTGATGTGATTGAAAAATCCGAGATCAGTAGCGAACTGGAAATCGACACCACCTGGGATGACGTTTACAG
TGCCAACTCTGGCAATACGGGCATCGCACTCGACGATGACATGCCGGTCTATCAAGGTGAAACGACGGAAACACTGCACG
ACTACTTACAGTGGCAACTGGATTTAACCCCGTTTAGCGATACTGACCGCATGATCGCCTTTGCCATCATTGATGCCGTC
GACGACACTGGCTACCTCACTATCAGCCCTGAAGAGATCTTAGAAAGCCTGAACAGCGTCGATATCGAACTCGACGAAGT
GGAAGCCGTACGCAAACGCATCCAACAGTTCGATCCCCTTGGTGTCGCGTCGCAAAACCTACAAGAATGCTTGCTGCTAC
AACTGGCCACATTTCCTATCGAGACCCCTTGGCTAGCCGAAGCCAAAATGGTCCTGAGCCATCACATTGATGCGCTAGGC
AATCGCGATTACAAGCTGATCGTCAAAGAAGCGAAGCTTAAAGAAGAAGACCTACGTGAAACGCTCAAACTGATCCAGCA
ACTTGATCCAAGGCCTGGCAGTAAGATCAATGCCATCGAGCCTGAGTACGTGATTCCAGATGTATCCGTATTTAAAGAGA
ATGGCAAATGGAGTGTCGCGATCAATCCAGACAGCATTCCAAAATTAAAAGTAAATCAACAGTATGCGCAACTAGGCAAA
GGCAGCAGCGCCGATAGCCAGTACATTCGCAGCAATTTGCAAGAGGCGAAATGGTTAATTAAGAGCCTTGAGAGCAGAAA
CGAGACGCTGCTCAAAGTTGCACGTTGCATAGTTGAACATCAACATGATTTCTTCGAGTATGGGGAAGAAGCAATGAAAC
CTATGGTGCTAAATGATGTGGCAACCGCGGTTGAGATGCATGAATCCACCATCTCACGCGTGACAACCCAGAAGTTTATG
CACACGCCTAGAGGTATCTTTGAATTGAAATATTTCTTCTCTAGCCATGTTAGTACGGATAGTGGAGGAGAATGTTCATC
GACAGCAATTCGCGCACTCATTAAGAAGTTAGTCACTGCCGAAAACAGTGCGAAACCACTGAGTGATAGCAAAATTGCTG
CCTTACTGGCAGACCAAGGAATCCAGGTCGCACGGCGAACGATAGCTAAGTACCGCGAGTCCTTAGGCATTGCCCCATCT
AGTCAGCGAAAACGCCTACTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

43.168

100

0.448