Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RW095_RS36940 Genome accession   NZ_CP136513
Coordinates   3179484..3180998 (-) Length   504 a.a.
NCBI ID   WP_317020644.1    Uniprot ID   -
Organism   Paraburkholderia kirstenboschensis strain F3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3174484..3185998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RW095_RS36915 (RW095_36915) - 3175346..3175981 (-) 636 WP_317020643.1 LON peptidase substrate-binding domain-containing protein -
  RW095_RS36920 (RW095_36920) rapZ 3176021..3176914 (-) 894 WP_128592435.1 RNase adapter RapZ -
  RW095_RS36925 (RW095_36925) hprK 3177018..3177986 (-) 969 WP_007179412.1 HPr(Ser) kinase/phosphatase -
  RW095_RS36930 (RW095_36930) - 3178126..3178641 (-) 516 WP_027804462.1 PTS sugar transporter subunit IIA -
  RW095_RS36935 (RW095_36935) hpf 3178956..3179312 (-) 357 WP_020066967.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RW095_RS36940 (RW095_36940) rpoN/rpoN1 3179484..3180998 (-) 1515 WP_317020644.1 RNA polymerase factor sigma-54 Machinery gene
  RW095_RS36945 (RW095_36945) lptB 3181178..3181963 (-) 786 WP_020066965.1 LPS export ABC transporter ATP-binding protein -
  RW095_RS36950 (RW095_36950) lptA 3181960..3182631 (-) 672 WP_128592271.1 lipopolysaccharide transport periplasmic protein LptA -
  RW095_RS36955 (RW095_36955) lptC 3182653..3183261 (-) 609 WP_317020645.1 LPS export ABC transporter periplasmic protein LptC -
  RW095_RS36960 (RW095_36960) - 3183258..3183818 (-) 561 WP_317020646.1 HAD family hydrolase -
  RW095_RS36965 (RW095_36965) kdsD 3183818..3184801 (-) 984 WP_317020647.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 504 a.a.        Molecular weight: 55293.38 Da        Isoelectric Point: 4.9891

>NTDB_id=891309 RW095_RS36940 WP_317020644.1 3179484..3180998(-) (rpoN/rpoN1) [Paraburkholderia kirstenboschensis strain F3]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAISQNPLLENEDDWIASPLRVAADGSLITQAPSSSAPPDQM
GGNGSSSSSSSERAENGEPQGVDEYNGLSGDSNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLCVTQ
AGQRDRALITFLIESLDDDGYLAATLDEVLADLPDELEVDLDELNAALALLHSFDPAGVGARSASECLKLQLLRLPPSPT
RTLALEIVAHYLELLAARDFTRLRKHLKANDDDLRDAHMLIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNPE
VVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIAD
TLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENAKTPLSDSRIAELLAEQGFV
VARRTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1515 bp        

>NTDB_id=891309 RW095_RS36940 WP_317020644.1 3179484..3180998(-) (rpoN/rpoN1) [Paraburkholderia kirstenboschensis strain F3]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGTTGACCCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCCACGCTCGAACTGCAGCAGGAAGTGGCAACCGCGATCTCGCAGAATCCGCTCCTCGAGAACGAGGACGACTGGA
TCGCGAGCCCGCTTCGCGTGGCGGCCGACGGTTCGCTGATTACGCAGGCGCCCAGTTCGTCAGCGCCGCCCGATCAGATG
GGCGGCAACGGCTCGTCATCGTCCAGCAGCAGTGAGCGCGCGGAGAACGGCGAGCCGCAAGGCGTGGACGAGTACAACGG
CCTCTCGGGCGACAGCAATGGCGACGCGTCGCAATGGAATCTCGACGACTACGGCCGCTCCGGCAACGCCTCCGACGACG
ACGACCTACCCCCGCTGCAAATTCACGAATCGAGTACTTCGCTGCGCGATCACCTGATGGCGCAACTGTGCGTTACGCAG
GCTGGTCAGCGTGACCGGGCGCTGATTACCTTCCTGATCGAGTCGCTAGACGACGACGGCTACCTTGCCGCCACGCTCGA
CGAAGTGCTCGCGGATCTGCCCGACGAACTCGAAGTCGACCTCGACGAACTGAATGCCGCGCTCGCGCTGCTGCATAGCT
TCGACCCGGCCGGCGTAGGCGCACGCTCCGCCTCTGAGTGTCTCAAGCTGCAACTGTTGCGGCTGCCGCCCTCGCCCACG
CGAACGCTCGCGCTGGAGATCGTTGCCCATTATCTGGAACTGCTGGCCGCACGCGATTTCACGCGTCTGCGCAAGCATCT
GAAAGCAAACGACGACGACCTGCGCGACGCGCACATGCTGATCCGCTCACTCGAACCGTTCCCTGGCGCAGCGTACGGCA
AGGCCGAAGCGGACTACGTGGTGCCGGACATCATGGTGCGTAAAACCGCCCAGGGCTGGCAGGCCGAGCTCAATCCCGAA
GTGGTGCCGAAGCTGCGCATCAATCACCTCTACGCCAACATTCTGCGCAACAACCGGGGCGATCCGGGCAGCGGTTCGTT
GCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAACGTTTCGAAACAATCCTGCGTGTCGCGCAAG
CAATTGTGGAGCGCCAGAAGAGCTTTTTTGTGCACGGCGAAATTGCTATGCGCCCCTTGGTTTTGCGAGAAATTGCTGAT
ACGCTGGGCCTACACGAGTCGACTGTCTCGCGTGTGACAACCGGTAAGTACATGCTCACCCCGTTCGGGACGCTGGAGTT
CAAGTACTTCTTCGGATCGCATGTTTCGACAGACACGGGCGGCGCGGCCTCTTCCACGGCCATTCGCGCGCTCATCAAGC
AACTGATAGGAGCGGAAAACGCAAAAACGCCTCTTTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTCGTG
GTCGCACGGCGCACCGTGGCGAAGTATCGCGAAGCACTCAGGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-331)

Sigma-54, DNA binding domain (Sigma54_DBD)

(347-502)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.211

100

0.673