Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RW095_RS36845 Genome accession   NZ_CP136513
Coordinates   3162869..3164050 (-) Length   393 a.a.
NCBI ID   WP_317020634.1    Uniprot ID   -
Organism   Paraburkholderia kirstenboschensis strain F3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3157869..3169050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RW095_RS36815 (RW095_36815) rpoH 3157919..3158854 (-) 936 WP_020067013.1 RNA polymerase sigma factor RpoH -
  RW095_RS36820 (RW095_36820) - 3159214..3159777 (+) 564 WP_317020629.1 acyloxyacyl hydrolase -
  RW095_RS36825 (RW095_36825) - 3159821..3160204 (+) 384 WP_317020630.1 nuclear transport factor 2 family protein -
  RW095_RS36830 (RW095_36830) ybiB 3160208..3161182 (-) 975 WP_317020631.1 DNA-binding protein YbiB -
  RW095_RS36835 (RW095_36835) - 3161408..3162106 (+) 699 WP_317020632.1 fumarylacetoacetate hydrolase family protein -
  RW095_RS36840 (RW095_36840) maiA 3162132..3162776 (+) 645 WP_317020633.1 maleylacetoacetate isomerase -
  RW095_RS36845 (RW095_36845) pilA 3162869..3164050 (-) 1182 WP_317020634.1 signal recognition particle-docking protein FtsY Machinery gene
  RW095_RS36850 (RW095_36850) rsmD 3164293..3164907 (+) 615 WP_317020635.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  RW095_RS36855 (RW095_36855) coaD 3165020..3165535 (+) 516 WP_288824630.1 pantetheine-phosphate adenylyltransferase -
  RW095_RS36860 (RW095_36860) - 3165599..3165856 (+) 258 WP_317020636.1 YfhL family 4Fe-4S dicluster ferredoxin -
  RW095_RS36865 (RW095_36865) hisC 3165925..3167004 (-) 1080 WP_317020637.1 histidinol-phosphate transaminase -
  RW095_RS36870 (RW095_36870) pth 3167169..3167771 (-) 603 WP_317020638.1 aminoacyl-tRNA hydrolase -
  RW095_RS36875 (RW095_36875) - 3167921..3168538 (-) 618 WP_128587852.1 50S ribosomal protein L25/general stress protein Ctc -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 41669.59 Da        Isoelectric Point: 4.6432

>NTDB_id=891308 RW095_RS36845 WP_317020634.1 3162869..3164050(-) (pilA) [Paraburkholderia kirstenboschensis strain F3]
MFSFFKRFKGSKESENAPDESQIAPEGLIDEPATEAPAAPPVPATTGTASTSSPPASIVPPTAASPEPQPQPDFEESTLE
TVEIVPPPLQDASAKGSWLTRLKTGLSKTSSSLTGIFVGTKIDEDLYEELETALIMSDAGVDATEFLLEALREKVRTERL
TEPQQVKTALRALLVELLKPLESSLMLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQSFNQSVLLAAGDTFRAAAREQLA
IWGQRNNVTVVSQESGDPAAVIFDAVGAARARKIDVMMADTAGRLPTQLHLMEELRKVKRVIGKAQDGAPHEVLLVIDAN
TGQNALAQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPIPVYFIGVGEKVEDLQPFSAEEFSDALLGG

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=891308 RW095_RS36845 WP_317020634.1 3162869..3164050(-) (pilA) [Paraburkholderia kirstenboschensis strain F3]
ATGTTCAGCTTTTTCAAACGATTCAAGGGTTCGAAAGAGTCCGAAAACGCGCCAGACGAATCGCAAATCGCGCCTGAGGG
GCTGATTGACGAGCCGGCTACAGAAGCGCCAGCCGCGCCTCCTGTCCCTGCAACCACCGGGACGGCTTCAACTTCCAGCC
CGCCGGCGAGCATTGTGCCGCCGACCGCCGCCTCGCCGGAACCGCAGCCTCAACCCGATTTCGAGGAATCGACGCTCGAG
ACCGTCGAGATCGTGCCTCCGCCCTTGCAGGATGCAAGCGCGAAAGGCTCGTGGCTGACTCGTCTGAAAACCGGCTTGTC
GAAGACCAGTTCGAGTCTCACCGGCATTTTCGTCGGCACCAAGATCGACGAGGATCTTTACGAAGAGCTGGAAACCGCGC
TGATCATGTCCGACGCGGGCGTCGACGCGACCGAATTCCTGCTTGAAGCGCTGCGCGAGAAGGTTCGCACCGAGCGTCTT
ACGGAGCCGCAACAGGTCAAGACGGCGCTGCGCGCGCTACTGGTCGAGTTGCTCAAGCCGCTGGAAAGCTCGCTGATGCT
CGGGCGCGCGCAGCCGCTCGTCATGATGATCGCGGGCGTCAACGGCGCGGGCAAGACGACGAGCATCGGCAAGCTCGCCA
AGCATCTGCAGAGTTTTAACCAGTCGGTGTTGCTTGCCGCCGGCGACACCTTCCGCGCCGCCGCCCGCGAGCAGTTGGCG
ATCTGGGGACAACGCAACAACGTCACCGTGGTGTCGCAGGAAAGCGGCGATCCGGCGGCCGTGATATTCGATGCGGTCGG
TGCCGCGCGGGCGCGCAAGATCGACGTGATGATGGCGGACACGGCCGGCCGTCTACCTACGCAGTTGCATCTAATGGAGG
AATTGCGCAAGGTCAAGCGCGTGATCGGCAAGGCGCAAGACGGCGCGCCGCACGAGGTGTTGCTCGTCATCGACGCCAAT
ACCGGGCAGAACGCGCTTGCGCAGGTCAAGGCTTTCGACGACGCGCTCGGCTTGACCGGCCTGATCGTCACCAAGCTCGA
CGGTACGGCCAAGGGCGGCATTCTTGCAGCCATCGCGCGGCAGCGACCAATTCCGGTGTACTTCATCGGCGTTGGCGAAA
AGGTCGAGGATTTGCAGCCTTTCAGCGCTGAAGAGTTCTCAGATGCGTTGCTAGGCGGGTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(193-391)

SRP54-type protein, helical bundle domain (SRP54_N)

(111-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.267

100

0.557