Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAGG05_RS03225 Genome accession   NZ_CP151934
Coordinates   667533..668966 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 8178 isolate T4048     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 662533..673966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGG05_RS03195 (AAGG05_03195) yrbG 663091..664068 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  AAGG05_RS03200 (AAGG05_03200) kdsD 664082..665068 (+) 987 WP_047086777.1 arabinose-5-phosphate isomerase KdsD -
  AAGG05_RS03205 (AAGG05_03205) kdsC 665089..665655 (+) 567 WP_000030015.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AAGG05_RS03210 (AAGG05_03210) lptC 665652..666227 (+) 576 WP_001539833.1 LPS export ABC transporter periplasmic protein LptC -
  AAGG05_RS03215 (AAGG05_03215) lptA 666196..666753 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AAGG05_RS03220 (AAGG05_03220) lptB 666760..667485 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  AAGG05_RS03225 (AAGG05_03225) rpoN/rpoN1 667533..668966 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  AAGG05_RS03230 (AAGG05_03230) hpf 668989..669276 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  AAGG05_RS03235 (AAGG05_03235) ptsN 669394..669885 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAGG05_RS03240 (AAGG05_03240) rapZ 669931..670785 (+) 855 WP_000243741.1 RNase adapter RapZ -
  AAGG05_RS03245 (AAGG05_03245) npr 670782..671054 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  AAGG05_RS03250 (AAGG05_03250) yrbL 671268..671900 (+) 633 WP_000620407.1 PhoP regulatory network protein YrbL -
  AAGG05_RS03255 (AAGG05_03255) mtgA 671897..672625 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AAGG05_RS03260 (AAGG05_03260) elbB 672622..673275 (-) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=891251 AAGG05_RS03225 WP_000809051.1 667533..668966(+) (rpoN/rpoN1) [Escherichia coli strain 8178 isolate T4048]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=891251 AAGG05_RS03225 WP_000809051.1 667533..668966(+) (rpoN/rpoN1) [Escherichia coli strain 8178 isolate T4048]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGATACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAAAGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAATATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTTTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465