Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   AAGG03_RS21750 Genome accession   NZ_CP151932
Coordinates   4432668..4434050 (+) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain 8178 isolate T4039     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4427668..4439050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGG03_RS21725 (AAGG03_21730) deoD 4428682..4429401 (+) 720 WP_000224879.1 purine-nucleoside phosphorylase -
  AAGG03_RS21730 (AAGG03_21735) - 4429562..4429825 (-) 264 WP_021531007.1 helix-turn-helix transcriptional regulator -
  AAGG03_RS21735 (AAGG03_21740) lplA 4429857..4430873 (-) 1017 WP_021520485.1 lipoate--protein ligase LplA -
  AAGG03_RS21740 (AAGG03_21745) ytjB 4430901..4431545 (-) 645 WP_000124608.1 YtjB family periplasmic protein -
  AAGG03_RS21745 (AAGG03_21750) serB 4431651..4432619 (+) 969 WP_001132964.1 phosphoserine phosphatase -
  AAGG03_RS21750 (AAGG03_21755) radA/sms 4432668..4434050 (+) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  AAGG03_RS21755 (AAGG03_21760) nadR 4434071..4435303 (+) 1233 WP_000093834.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  AAGG03_RS21760 (AAGG03_21765) - 4435395..4435727 (+) 333 WP_000513550.1 type II toxin-antitoxin system RelE/ParE family toxin -
  AAGG03_RS21765 (AAGG03_21770) nadS 4435729..4436013 (+) 285 WP_000007436.1 NadS family protein -
  AAGG03_RS21770 (AAGG03_21775) ettA 4436069..4437736 (-) 1668 WP_000046754.1 energy-dependent translational throttle protein EttA -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=891226 AAGG03_RS21750 WP_001029698.1 4432668..4434050(+) (radA/sms) [Escherichia coli strain 8178 isolate T4039]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=891226 AAGG03_RS21750 WP_001029698.1 4432668..4434050(+) (radA/sms) [Escherichia coli strain 8178 isolate T4039]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCAGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGAGTGGCAAAAGTCCAGAAACTTTCCGACATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGCGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCGCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTTAATATGCTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGCAGCGTGGCACAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTCGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTGGATGGTGATGCCGACTCACGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGTGTCTTCGCAATGACCGAGCAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTAGTGTGGGAAGGAACGCGTCCGCTGCTGGTGGAGATTCAGGCGCTCGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTGGCAGTCGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTTCACCG
TCACGGTGGTCTACAAATGGCCGATCAGGATGTGTTTGTTAACGTGGTCGGTGGCGTGAAGGTGACGGAAACCAGTGCCG
ATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTACCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAACGAATCTCAGAAGCGGCGAAACACGGTTTTCGCCGGGC
AATTGTTCCGGCAGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43