Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AAGG04_RS13375 Genome accession   NZ_CP151931
Coordinates   2688455..2688895 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain 8178 isolate T2213     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2683455..2693895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGG04_RS13355 (AAGG04_13330) aroP 2684455..2685825 (+) 1371 WP_001540704.1 aromatic amino acid transporter AroP -
  AAGG04_RS13360 (AAGG04_13335) ampE 2685868..2686722 (-) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  AAGG04_RS13365 (AAGG04_13340) ampD 2686719..2687270 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AAGG04_RS13370 (AAGG04_13345) nadC 2687358..2688251 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAGG04_RS13375 (AAGG04_13350) pilA 2688455..2688895 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  AAGG04_RS13380 (AAGG04_13355) pilB 2688905..2690290 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  AAGG04_RS13385 (AAGG04_13360) hofC 2690280..2691482 (+) 1203 WP_001540700.1 protein transport protein HofC -
  AAGG04_RS13390 (AAGG04_13365) guaC 2691517..2692560 (-) 1044 WP_001217338.1 GMP reductase -
  AAGG04_RS13395 (AAGG04_13370) - 2692716..2692760 (-) 45 WP_120795372.1 protein YacM -
  AAGG04_RS13400 (AAGG04_13375) coaE 2692785..2693405 (+) 621 WP_001269524.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=891096 AAGG04_RS13375 WP_000360914.1 2688455..2688895(+) (pilA) [Escherichia coli strain 8178 isolate T2213]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=891096 AAGG04_RS13375 WP_000360914.1 2688455..2688895(+) (pilA) [Escherichia coli strain 8178 isolate T2213]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363