Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   WLF14_RS28820 Genome accession   NZ_CP151816
Coordinates   6323679..6324044 (-) Length   121 a.a.
NCBI ID   WP_014720320.1    Uniprot ID   A0A0J8G142
Organism   Pseudomonas fluorescens strain IMGN2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6318679..6329044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WLF14_RS28810 (WLF14_28810) - 6321094..6323145 (-) 2052 WP_024077895.1 methyl-accepting chemotaxis protein -
  WLF14_RS28815 (WLF14_28815) - 6323142..6323666 (-) 525 WP_024077896.1 chemotaxis protein CheW -
  WLF14_RS28820 (WLF14_28820) pilH 6323679..6324044 (-) 366 WP_014720320.1 twitching motility response regulator PilH Machinery gene
  WLF14_RS28825 (WLF14_28825) pilG 6324091..6324498 (-) 408 WP_010564379.1 twitching motility response regulator PilG Regulator
  WLF14_RS28830 (WLF14_28830) gshB 6324738..6325691 (+) 954 WP_024077897.1 glutathione synthase -
  WLF14_RS28835 (WLF14_28835) - 6325769..6326674 (+) 906 WP_024077898.1 energy transducer TonB -
  WLF14_RS28840 (WLF14_28840) - 6326756..6327325 (+) 570 WP_024077899.1 YqgE/AlgH family protein -
  WLF14_RS28845 (WLF14_28845) ruvX 6327325..6327762 (+) 438 WP_342308500.1 Holliday junction resolvase RuvX -
  WLF14_RS28850 (WLF14_28850) pyrR 6327846..6328352 (+) 507 WP_024077900.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 121 a.a.        Molecular weight: 13227.23 Da        Isoelectric Point: 4.9105

>NTDB_id=890969 WLF14_RS28820 WP_014720320.1 6323679..6324044(-) (pilH) [Pseudomonas fluorescens strain IMGN2]
MARVLIVDDSPTEMYKLTGMLEKHGHQVLKAENGADGVALARQEKPDAVLMDIVMPGLNGFQATRQLSKEPETNGIPIII
ITTKDQETDKIWGARQGAKDYLTKPVDEETLIATLNKVLAG

Nucleotide


Download         Length: 366 bp        

>NTDB_id=890969 WLF14_RS28820 WP_014720320.1 6323679..6324044(-) (pilH) [Pseudomonas fluorescens strain IMGN2]
ATGGCACGCGTTCTGATCGTCGACGATTCGCCGACTGAAATGTACAAACTGACCGGTATGCTGGAAAAGCACGGCCACCA
GGTCCTCAAGGCCGAAAACGGCGCAGACGGTGTGGCCCTGGCTCGCCAGGAAAAGCCCGACGCGGTCCTGATGGACATCG
TGATGCCGGGCCTCAATGGTTTCCAGGCGACCCGCCAGCTGTCCAAGGAGCCGGAAACCAACGGTATTCCGATCATCATC
ATCACCACCAAGGACCAGGAGACCGACAAGATCTGGGGCGCGCGCCAAGGCGCCAAGGACTACCTGACCAAGCCGGTGGA
TGAAGAAACCCTGATCGCCACACTGAACAAGGTGTTGGCCGGCTGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J8G142

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

36.975

98.347

0.364