Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MHOCP_RS12400 Genome accession   NZ_CP136421
Coordinates   2384924..2386282 (-) Length   452 a.a.
NCBI ID   WP_273173988.1    Uniprot ID   -
Organism   Moorella humiferrea strain OCP     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2379924..2391282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHOCP_RS12375 (MHOCP_24690) cysS 2380140..2381570 (-) 1431 WP_338824375.1 cysteine--tRNA ligase -
  MHOCP_RS12380 (MHOCP_24700) cysE 2381515..2382225 (-) 711 WP_106005291.1 serine O-acetyltransferase -
  MHOCP_RS12385 (MHOCP_24710) ispF 2382527..2383024 (-) 498 WP_338824377.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MHOCP_RS12390 (MHOCP_24720) ispD 2383009..2383707 (-) 699 WP_338824378.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MHOCP_RS12395 (MHOCP_24730) - 2383695..2384858 (-) 1164 WP_106005294.1 PIN/TRAM domain-containing protein -
  MHOCP_RS12400 (MHOCP_24740) radA 2384924..2386282 (-) 1359 WP_273173988.1 DNA repair protein RadA Machinery gene
  MHOCP_RS12405 (MHOCP_24750) - 2386940..2388379 (-) 1440 WP_338824379.1 ATP-binding protein -
  MHOCP_RS12410 (MHOCP_24760) - 2388394..2389599 (-) 1206 WP_338824380.1 HD domain-containing phosphohydrolase -
  MHOCP_RS12415 (MHOCP_24770) - 2389668..2389880 (-) 213 WP_106005299.1 DUF1858 domain-containing protein -
  MHOCP_RS12420 (MHOCP_24780) - 2389953..2390261 (-) 309 WP_106005300.1 MTH1187 family thiamine-binding protein -
  MHOCP_RS12430 (MHOCP_24800) - 2390476..2391183 (-) 708 WP_338824382.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48472.18 Da        Isoelectric Point: 6.5037

>NTDB_id=890912 MHOCP_RS12400 WP_273173988.1 2384924..2386282(-) (radA) [Moorella humiferrea strain OCP]
MAKIKERFICQVCGYETWSYLGRCPSCGSWNSLVVEVVAGEMVKGGGRKEAVPSLLTEIADIKGERLVSMLGEWDRVLGG
GLVPGSLILVGGAPGIGKSTLLLQVAHSLAERYGKILYISGEESPEQVKLRAQRLKALKGPIYFLAETDVEGVLAGIEGL
DPVVAIIDSIQTMYLPAIQAAPGSVSQVRECAARFQRLAKKGGTAVILVGHVTKEGFLAGPRVLEHLVDTVLYLEGERYQ
AYRILRAAKNRFGSTNEIGIFEMDARGLKEVANPSETLLAERPVGVAGSSVAACMEGTRPLLLEIQALVSPTSFGNPRRL
TTGVDYNRALLLTAVLEKRAGLNLAGHDVYLNVAGGITINEPAADLAICLAIASGFKDRPVAQETLVLGEVGLAGEVRAV
SQLERRIEEAIRLGFKRFIIPAGNKSSLFSQNDCEFYSVRSVIEALEVGLEI

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=890912 MHOCP_RS12400 WP_273173988.1 2384924..2386282(-) (radA) [Moorella humiferrea strain OCP]
TTGGCCAAAATAAAGGAGCGTTTTATCTGCCAGGTATGCGGTTACGAAACATGGAGCTACCTGGGCCGCTGCCCTTCCTG
CGGCAGTTGGAATAGTTTGGTGGTAGAAGTTGTAGCGGGAGAAATGGTGAAGGGAGGAGGCAGGAAGGAGGCAGTCCCCT
CCCTTTTGACGGAAATCGCCGATATTAAGGGTGAGCGTCTGGTCAGTATGCTGGGGGAATGGGACCGGGTCCTCGGGGGT
GGACTGGTTCCGGGCTCTTTGATCCTCGTCGGCGGCGCACCGGGCATCGGCAAATCCACCCTTCTTTTACAGGTAGCCCA
CAGCCTCGCCGAAAGGTACGGCAAAATCCTTTATATTTCAGGGGAAGAATCCCCGGAGCAGGTAAAACTCCGGGCCCAGC
GACTGAAGGCATTGAAGGGGCCGATATATTTCCTGGCCGAAACGGATGTCGAAGGTGTACTGGCCGGAATTGAAGGACTG
GACCCGGTAGTAGCCATCATTGATTCAATTCAAACAATGTACCTTCCGGCCATCCAGGCGGCACCGGGTAGTGTTTCCCA
GGTCCGGGAGTGTGCGGCGCGCTTTCAGCGCCTGGCCAAAAAAGGAGGCACGGCCGTCATCCTCGTCGGCCATGTTACCA
AAGAAGGTTTTCTGGCTGGCCCGCGCGTCCTTGAACACTTGGTGGATACCGTCCTCTACCTTGAAGGAGAACGCTACCAG
GCCTACCGTATCCTCCGGGCGGCTAAAAATCGCTTCGGTTCGACAAATGAAATCGGCATATTTGAAATGGATGCCCGGGG
GCTGAAAGAGGTGGCCAATCCTTCGGAAACCCTTTTGGCCGAACGGCCGGTCGGCGTTGCCGGCTCCAGTGTGGCGGCCT
GCATGGAAGGAACGCGGCCTCTGCTGCTGGAAATCCAGGCCCTGGTAAGCCCGACATCCTTCGGCAACCCCCGGCGGCTG
ACTACCGGCGTCGATTATAACCGTGCCCTGCTCCTTACGGCGGTCCTGGAAAAGCGGGCGGGCCTAAATTTGGCTGGCCA
CGACGTCTATCTCAATGTCGCCGGGGGCATTACTATAAATGAACCGGCCGCCGACCTGGCTATCTGTCTGGCCATAGCTT
CCGGTTTTAAAGACAGACCTGTAGCCCAAGAAACATTAGTTTTGGGGGAGGTGGGTCTGGCTGGGGAAGTCCGGGCCGTA
AGCCAGCTGGAGAGACGTATCGAAGAAGCCATCCGCCTGGGCTTTAAACGCTTTATAATACCCGCGGGAAATAAAAGCAG
CCTTTTTTCCCAGAATGATTGCGAATTTTATAGCGTGCGGTCGGTGATTGAGGCCTTAGAAGTCGGCCTGGAAATTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-216)

Subunit ChlI of Mg-chelatase (ChlI)

(340-424)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

54.783

100

0.558
  radA Streptococcus mitis SK321

53.215

99.779

0.531
  radA Streptococcus pneumoniae Rx1

53.215

99.779

0.531
  radA Streptococcus pneumoniae D39

53.215

99.779

0.531
  radA Streptococcus pneumoniae R6

53.215

99.779

0.531
  radA Streptococcus pneumoniae TIGR4

53.215

99.779

0.531
  radA Streptococcus mitis NCTC 12261

52.993

99.779

0.529