Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WLF14_RS04825 Genome accession   NZ_CP151816
Coordinates   1065968..1067461 (-) Length   497 a.a.
NCBI ID   WP_024073469.1    Uniprot ID   A0ACA7P1E1
Organism   Pseudomonas fluorescens strain IMGN2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1060968..1072461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WLF14_RS04790 (WLF14_04790) - 1061957..1062385 (+) 429 WP_024073462.1 hypothetical protein -
  WLF14_RS04795 (WLF14_04795) - 1062387..1062998 (+) 612 WP_024073463.1 superoxide dismutase -
  WLF14_RS04800 (WLF14_04800) - 1063032..1063925 (+) 894 WP_024073464.1 ZIP family metal transporter -
  WLF14_RS04805 (WLF14_04805) - 1063961..1064233 (-) 273 WP_024073465.1 HPr family phosphocarrier protein -
  WLF14_RS04810 (WLF14_04810) rapZ 1064250..1065107 (-) 858 WP_024073466.1 RNase adapter RapZ -
  WLF14_RS04815 (WLF14_04815) ptsN 1065110..1065574 (-) 465 WP_069075268.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WLF14_RS04820 (WLF14_04820) raiA 1065587..1065892 (-) 306 WP_024073468.1 ribosome-associated translation inhibitor RaiA -
  WLF14_RS04825 (WLF14_04825) rpoN/rpoN1 1065968..1067461 (-) 1494 WP_024073469.1 RNA polymerase factor sigma-54 Machinery gene
  WLF14_RS04830 (WLF14_04830) lptB 1067647..1068372 (-) 726 WP_003171806.1 LPS export ABC transporter ATP-binding protein -
  WLF14_RS04835 (WLF14_04835) lptA 1068372..1068905 (-) 534 WP_024073470.1 lipopolysaccharide transport periplasmic protein LptA -
  WLF14_RS04840 (WLF14_04840) lptC 1068892..1069464 (-) 573 WP_024073471.1 LPS export ABC transporter periplasmic protein LptC -
  WLF14_RS04845 (WLF14_04845) - 1069473..1069997 (-) 525 WP_069075269.1 HAD family hydrolase -
  WLF14_RS04850 (WLF14_04850) - 1069998..1070972 (-) 975 WP_024073473.1 KpsF/GutQ family sugar-phosphate isomerase -
  WLF14_RS04855 (WLF14_04855) - 1071285..1072094 (+) 810 WP_024073474.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56578.67 Da        Isoelectric Point: 4.3878

>NTDB_id=890901 WLF14_RS04825 WP_024073469.1 1065968..1067461(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain IMGN2]
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEEGDDFDNADPLADNIEQKPNPDVQEPSYQ
ETAPTVDNLEEGDWNERIPNELPVDTAWEDVYQTSASSLPSNDDDEWDFTTRTSAGESLQSHLLWQLNLAPMSDTDRLIA
VTLIDCINNQGYLDETLDEILEAFDPELDIELDEIEAVLHRIQQFEPAGIGARTLSECLLLQLRQLPAKTPWLAEAQRLV
TDYIDLLGSRDYSQLMRRMKLKEDDLRQVIELVQSLNPRPGSQIESSEAEYVVPDVIVRKDNERWLVELNQESVPRLRVN
PQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHEST
ISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLM

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=890901 WLF14_RS04825 WP_024073469.1 1065968..1067461(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain IMGN2]
ATGAAACCATCGCTAGTCCTGAGAATGGGCCAGCAGCTGACGATGACACCGCAGCTGCAACAGGCCATCCGCCTGCTCCA
ATTGTCGACCCTGGACCTGCAACAGGAAATCCAGGAGGCCCTGGAGTCCAATCCGATGCTCGAACGCCAGGAAGAAGGCG
ACGACTTCGACAATGCAGACCCGCTGGCCGACAACATCGAGCAAAAACCCAACCCCGATGTGCAGGAACCCTCCTATCAG
GAAACCGCCCCGACGGTGGATAACCTCGAGGAAGGCGACTGGAACGAACGCATACCCAACGAGCTGCCCGTGGACACCGC
CTGGGAAGACGTCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGATGATGACGAGTGGGACTTCACCACCCGCACGT
CCGCCGGCGAGAGCCTGCAGAGCCACCTGTTGTGGCAATTGAACCTCGCGCCGATGTCCGACACCGATCGCCTGATCGCC
GTGACCCTGATCGACTGCATCAACAACCAGGGTTACCTGGACGAAACGCTCGACGAGATTCTTGAAGCCTTCGATCCGGA
ACTGGATATCGAACTGGACGAGATCGAAGCCGTCCTGCACCGCATCCAGCAATTCGAGCCCGCCGGCATTGGCGCGCGCA
CCCTCAGCGAGTGCCTGCTGCTGCAACTGCGTCAACTGCCTGCCAAGACGCCTTGGCTTGCCGAAGCCCAACGCCTGGTC
ACGGACTACATCGACCTGCTGGGCAGCCGCGACTACAGCCAGTTGATGCGCCGTATGAAGCTCAAGGAAGACGACCTGCG
CCAGGTGATCGAGCTGGTACAGAGCCTCAACCCTCGCCCGGGCTCGCAGATCGAGTCCAGCGAAGCCGAATACGTCGTCC
CCGACGTGATCGTACGCAAGGACAACGAGCGCTGGCTGGTGGAACTGAACCAGGAGTCGGTACCGCGCCTGCGGGTCAAT
CCGCAATATGCCGGTTTTGTGCGTCGCGCCGACACCAGCGCCGACAACACCTTCATGCGCAACCAGTTGCAGGAAGCGCG
CTGGTTCATCAAGAGCCTGCAAAGCCGCAACGAAACCCTGATGAAAGTCGCCACCCAGATCGTCGAGCACCAGCGTGGCT
TCCTGGAATACGGCGACGAAGCGATGAAGCCTCTGGTCCTGCATGACATCGCCGAAGCGGTTGGCATGCATGAGTCAACG
ATTTCCCGGGTGACCACGCAAAAATTCATGCATACCCCACGGGGCATATATGAGCTGAAATACTTTTTCTCCAGCCATGT
CAGCACCTCCGAAGGCGGCGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAAAAACTGGTTGCGGCGGAAAATCAGA
AAAAGCCGTTGAGTGACAGCAAGATCGCTGGTTTACTGGAGGCACAAGGCATTCAGGTCGCCCGTCGAACCGTCGCCAAG
TACCGCGAGTCCCTTGGGATCGCGCCTTCCAGCGAGCGTAAGCGTTTGATGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.664

100

0.455