Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MCACP_RS13995 Genome accession   NZ_CP136420
Coordinates   2741503..2742861 (-) Length   452 a.a.
NCBI ID   WP_406678762.1    Uniprot ID   -
Organism   Moorella sp. ACPs     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2736503..2747861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCACP_RS13970 (MCACP_27910) cysS 2736577..2738007 (-) 1431 WP_406678761.1 cysteine--tRNA ligase -
  MCACP_RS13975 (MCACP_27920) cysE 2737955..2738671 (-) 717 WP_406677345.1 serine O-acetyltransferase -
  MCACP_RS13980 (MCACP_27930) ispF 2738970..2739461 (-) 492 WP_406677346.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MCACP_RS13985 (MCACP_27940) ispD 2739461..2740150 (-) 690 WP_406677347.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MCACP_RS13990 (MCACP_27950) - 2740138..2741289 (-) 1152 WP_406677348.1 PIN/TRAM domain-containing protein -
  MCACP_RS13995 (MCACP_27960) radA 2741503..2742861 (-) 1359 WP_406678762.1 DNA repair protein RadA Machinery gene
  MCACP_RS14000 (MCACP_27970) - 2742883..2743668 (-) 786 WP_406677349.1 phosphatase -
  MCACP_RS14005 (MCACP_27980) pnpS 2744416..2745915 (-) 1500 WP_406677350.1 two-component system histidine kinase PnpS -
  MCACP_RS14010 (MCACP_27990) - 2745919..2747121 (-) 1203 WP_406677351.1 HD-GYP domain-containing protein -
  MCACP_RS14015 (MCACP_28000) - 2747194..2747388 (-) 195 WP_406678763.1 DUF1858 domain-containing protein -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 47943.62 Da        Isoelectric Point: 6.1756

>NTDB_id=890894 MCACP_RS13995 WP_406678762.1 2741503..2742861(-) (radA) [Moorella sp. ACPs]
MAKTKERFVCQQCGYESLSFLGRCPACGSWNSLVAEVLPLEGISRATGLKASPALLADISDITGERLISTLGEWDRVVGG
GLVPGSLILVGGAPGIGKSTLLLQVGQRLAARYGKILYVSGEESPGQVRLRAQRLDALHGEIYFLAETDVEAILAGIESL
SPVAVIVDSIQTMFLPAIQAAPGSVSQVRECAARFLRLAKGGGPAVILVGHVTKEGFLAGPRVLEHLVDTVLYLEGERYQ
AYRILRAAKNRFGSTNEIGIFEMVTGGLKEVANPSELLLAERPAGVAGSSVVACLEGTRPLLLEIQALVSPTTFGNPRRL
ATGVDFNRALLLTAVLEKRAGLHLGGYDIYLNVAGGIAINEPAADLAICLAIASGLKDRPVEAETLVLGEVGLAGEVRAI
GQVERRIEEAARLGFKRFLIPAGNKSRLINQSDYEIYAVRSVVEALELGLMV

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=890894 MCACP_RS13995 WP_406678762.1 2741503..2742861(-) (radA) [Moorella sp. ACPs]
CTGGCGAAAACAAAAGAGCGTTTTGTCTGCCAGCAATGTGGTTATGAAAGCTTAAGTTTTTTAGGCCGCTGCCCGGCCTG
CGGCAGCTGGAACAGCCTGGTAGCAGAAGTTTTACCCCTGGAAGGAATAAGCCGCGCGACCGGATTGAAAGCGTCTCCCG
CCCTGCTGGCAGATATTTCCGATATTACCGGTGAGCGTTTGATAAGTACTTTGGGTGAATGGGACCGGGTAGTGGGTGGT
GGCCTGGTACCGGGTTCTCTAATTCTGGTCGGCGGGGCGCCGGGCATTGGCAAGTCGACCTTACTCCTCCAGGTGGGCCA
AAGGCTTGCCGCCAGGTACGGCAAGATCCTCTATGTTTCCGGCGAAGAATCTCCCGGGCAGGTCAGGCTCAGAGCCCAGC
GCCTGGACGCCCTCCACGGCGAGATCTATTTCCTGGCCGAGACGGATGTAGAAGCCATCCTGGCCGGGATAGAAAGTTTA
AGCCCGGTAGCAGTCATTGTAGATTCCATTCAAACCATGTTCCTGCCCGCTATCCAGGCAGCGCCAGGGAGCGTATCCCA
GGTGCGGGAATGTGCGGCGCGTTTCTTGCGCCTGGCAAAAGGTGGCGGCCCGGCCGTAATCCTGGTCGGTCATGTCACCA
AAGAAGGATTTCTGGCCGGTCCCCGGGTCCTGGAACATCTGGTGGACACTGTCCTCTACCTGGAAGGGGAACGTTATCAG
GCTTACCGGATCCTGCGTGCTGCCAAAAATCGTTTTGGTTCTACCAATGAAATCGGGATTTTTGAGATGGTTACCGGGGG
GCTCAAGGAAGTGGCCAACCCATCGGAGCTGCTCCTGGCCGAACGGCCGGCCGGTGTTGCCGGCTCCAGTGTGGTGGCCT
GTTTGGAAGGGACCCGTCCCTTACTCCTGGAGATCCAGGCCCTGGTAAGCCCGACCACCTTTGGCAACCCGCGGCGGCTG
GCCACAGGGGTCGATTTTAACCGCGCCCTGCTGCTTACGGCAGTCCTGGAAAAAAGGGCGGGATTACACCTGGGCGGCTA
TGACATTTACCTTAACGTCGCCGGGGGCATCGCCATCAATGAGCCGGCAGCCGACCTTGCCATATGCCTGGCCATAGCCT
CCGGCCTTAAAGACCGGCCGGTAGAGGCCGAAACTTTAGTTTTAGGGGAGGTCGGCCTGGCAGGTGAAGTCCGGGCCATA
GGCCAGGTTGAAAGGCGTATAGAAGAAGCAGCCAGGTTGGGTTTCAAGCGTTTTCTTATCCCGGCGGGGAATAAGAGCCG
TCTTATAAACCAGAGTGATTACGAAATCTATGCTGTACGGTCAGTGGTTGAGGCCCTGGAACTCGGTTTGATGGTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

Subunit ChlI of Mg-chelatase (ChlI)

(343-424)

AAA domain (AAA_25)

(75-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

54.167

100

0.546
  radA Streptococcus pneumoniae Rx1

53.556

99.558

0.533
  radA Streptococcus pneumoniae D39

53.556

99.558

0.533
  radA Streptococcus pneumoniae R6

53.556

99.558

0.533
  radA Streptococcus pneumoniae TIGR4

53.556

99.558

0.533
  radA Streptococcus mitis SK321

53.556

99.558

0.533
  radA Streptococcus mitis NCTC 12261

53.333

99.558

0.531