Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   WLF14_RS02285 Genome accession   NZ_CP151816
Coordinates   503269..505488 (-) Length   739 a.a.
NCBI ID   WP_024072956.1    Uniprot ID   A0ACA7NZD4
Organism   Pseudomonas fluorescens strain IMGN2     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498269..510488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WLF14_RS02265 (WLF14_02265) hslU 498556..499893 (-) 1338 WP_024072953.1 ATP-dependent protease ATPase subunit HslU -
  WLF14_RS02270 (WLF14_02270) hslV 499931..500461 (-) 531 WP_010563201.1 ATP-dependent protease subunit HslV -
  WLF14_RS02275 (WLF14_02275) - 500719..501420 (-) 702 WP_024072954.1 SPOR domain-containing protein -
  WLF14_RS02280 (WLF14_02280) argS 501422..503158 (-) 1737 WP_024072955.1 arginine--tRNA ligase -
  WLF14_RS02285 (WLF14_02285) priA 503269..505488 (-) 2220 WP_024072956.1 primosomal protein N' Machinery gene
  WLF14_RS02290 (WLF14_02290) rpmE 505769..506002 (+) 234 WP_005784030.1 50S ribosomal protein L31 -
  WLF14_RS02295 (WLF14_02295) - 506017..506823 (+) 807 WP_342308807.1 thermonuclease family protein -
  WLF14_RS02300 (WLF14_02300) - 507005..508273 (+) 1269 WP_024072958.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81964.58 Da        Isoelectric Point: 8.4931

>NTDB_id=890892 WLF14_RS02285 WP_024072956.1 503269..505488(-) (priA) [Pseudomonas fluorescens strain IMGN2]
MPDAILRLALPSPLRRLFDYRAPAGVLRAQLQPGMRVRVPFGRREMIGILVEVADHSEVPAEKLKPAVAILDATAPLPPA
LFKLCLWTAQYYQHSLGDTLSWALPVLLRQGEPAEARQERFWSMVPGARLDDPRIARAPRQREALATLAQHPHGVAHQLL
SKLMLSKDSLDLLLAKGLVQVEIRKHAPDPRHEHWLAQPELPLNPEQRAAYEAIRAGFDCFHAFLLAGVTGSGKTEVYLQ
LIRETLQAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLESWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQEDIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECERCDARMTVHQRHGELRCHHCGHVERVPRHC
PQCGKVDLRPVGAGTERAEERLGILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFAHL
ALLRAEAHKPGQAEGFLDEACSAAERLLGELGLGGIELLGPVPAPMERRAGRYRAQLLLQAISRAPLHRLLSSWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=890892 WLF14_RS02285 WP_024072956.1 503269..505488(-) (priA) [Pseudomonas fluorescens strain IMGN2]
GTGCCCGACGCCATCCTGCGCCTCGCCCTGCCCTCGCCCCTGCGCCGCCTGTTCGACTACCGGGCCCCGGCCGGTGTGCT
GCGCGCGCAGTTGCAGCCGGGCATGCGCGTGCGCGTGCCGTTTGGCCGCAGGGAGATGATCGGCATCCTGGTGGAAGTCG
CCGACCACAGCGAAGTGCCCGCCGAAAAACTCAAGCCCGCCGTGGCCATCCTTGATGCCACGGCGCCTCTGCCGCCCGCG
CTGTTCAAGCTGTGCCTGTGGACCGCCCAGTATTACCAGCACAGCCTGGGCGACACATTGAGCTGGGCGCTGCCCGTACT
GCTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGGAGCGCTTCTGGTCGATGGTGCCCGGCGCGCGCCTTGACGACCCAC
GCATCGCCCGTGCCCCGCGCCAGCGTGAAGCCCTCGCCACCCTGGCCCAGCACCCCCATGGCGTCGCGCATCAGCTATTA
AGCAAGCTGATGCTGAGCAAAGACAGCCTCGACCTGCTGCTGGCCAAGGGCCTGGTGCAAGTGGAAATCCGCAAGCACGC
CCCCGACCCACGCCACGAACACTGGCTGGCCCAGCCGGAATTGCCGCTCAACCCCGAACAACGCGCCGCGTACGAAGCCA
TTCGCGCCGGTTTCGACTGTTTCCACGCATTCCTGCTGGCCGGTGTCACCGGCAGCGGCAAGACCGAAGTCTATTTGCAG
CTGATCCGCGAAACCCTGCAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCCGAAATCAACCTGGGCCCGCAGACCCTGGC
GCGTTTCGAGCAACGCTTCAACGCGCGTATCGCCCTGGTGCATTCGGCGGTCAACGACCGGGAGCGCCTGGAGTCCTGGC
TGGCGGCGCGGGACGGCGAAGCAGACATCATTATCGGCACCCGTTCGGCGCTGTTCACGCCGATGAAAAATCCCGGGCTG
ATCATCATCGACGAGGAGCACGACGGCTCCTATAAACAACAGGAAGGCCTGCGCTACCACGCCCGCGACCTGGCGCTGGT
GCGCGCCCGCCAGGAAGACATCCCGATCGTGCTGGGTTCAGCCACGCCGTCCCTGGAAAGCCTGCACAACGCCTACACCG
GTCGTTATGGACTGCTGCGCTTGAATGAACGGGCCGGCGGCGCCAAACAGCCCCGCTTCCTGCGCCTGGACGTGAAAAGC
CGACCGCTGGACAGCGGGATTTCCGGCCCGATGCAGCAGGCCATCGGCCAGACCCTCGCTGCCGGCCAGCAAGTCCTGGT
GTTTCTCAACCGCCGTGGCTTTGCGCCGACGCTGCTGTGCCATGACTGCGGCTGGATGTCCGAGTGCGAGCGCTGCGATG
CGCGCATGACCGTCCACCAGCGCCATGGCGAACTACGCTGCCACCACTGCGGCCATGTGGAGCGCGTGCCACGCCACTGC
CCGCAGTGCGGCAAGGTCGACCTGCGCCCGGTGGGTGCCGGCACTGAGCGCGCCGAAGAGCGCCTGGGCATTCTGTTCCC
GGACTACCCGGTACTGCGGGTCGATCGCGACAGCACCTCGCGCAAAGACGCGATGAACCAACTGTTCGCCACGATCCAGA
AAGGCCAGCCGTGCATCCTGGTGGGCACGCAAATGCTTGCCAAAGGCCATCACTTTCCCCGGGTGACCCTGGTATCGATC
CTGGATGCCGATGGCGGCCTGTTTTCCGGCGATTTCCGCGCCAGCGAGCGCATGGCACAACTGATCGTGCAGGTCGCCGG
GCGCGCCGGGCGGGCTGAGGAGCCGGGCAAGGTGATCATCCAGACACACCTGGCCGACCATCCACTGCTGATTCAACTGA
CAGAACAAGGCTACTTCGCCTTCGCCGAGCAGGCGCTGAGTGAACGCCGCTCCGCTGGCCTGCCGCCCTTTGCGCACCTG
GCGTTGCTGCGCGCCGAAGCCCACAAGCCGGGGCAGGCCGAAGGTTTTCTGGATGAAGCGTGCAGCGCTGCCGAACGCTT
GCTCGGCGAACTGGGCCTGGGCGGCATCGAATTGCTCGGCCCCGTGCCCGCCCCCATGGAGCGCCGCGCCGGACGTTATC
GCGCTCAACTACTCCTGCAAGCCATCTCCCGCGCGCCCCTGCACCGGCTATTAAGTAGCTGGTTACTTGCGCTGGAGCAA
ATGCCCAGCGGCCGCCAGGTGCGATGGTCACTGGATGTAGACCCGGTAGATTTGTATTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(205-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.241

100

0.475
  priA Bacillus subtilis subsp. subtilis str. 168

34.625

100

0.375