Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MHFGQ_RS13720 Genome accession   NZ_CP136419
Coordinates   2642118..2643476 (-) Length   452 a.a.
NCBI ID   WP_245907827.1    Uniprot ID   A0A2T0AS51
Organism   Moorella humiferrea strain 64-FGQ     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2637118..2648476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHFGQ_RS13695 (MHFGQ_27280) cysS 2637334..2638761 (-) 1428 WP_106005290.1 cysteine--tRNA ligase -
  MHFGQ_RS13700 (MHFGQ_27290) cysE 2638709..2639419 (-) 711 WP_106005291.1 serine O-acetyltransferase -
  MHFGQ_RS13705 (MHFGQ_27300) ispF 2639721..2640218 (-) 498 WP_106005292.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MHFGQ_RS13710 (MHFGQ_27310) ispD 2640203..2640901 (-) 699 WP_106005293.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MHFGQ_RS13715 (MHFGQ_27320) - 2640889..2642052 (-) 1164 WP_106005294.1 PIN/TRAM domain-containing protein -
  MHFGQ_RS13720 (MHFGQ_27330) radA 2642118..2643476 (-) 1359 WP_245907827.1 DNA repair protein RadA Machinery gene
  MHFGQ_RS13725 (MHFGQ_27340) - 2644134..2645573 (-) 1440 WP_106005297.1 sensor histidine kinase -
  MHFGQ_RS13730 (MHFGQ_27350) - 2645588..2646793 (-) 1206 WP_106005298.1 HD-GYP domain-containing protein -
  MHFGQ_RS13735 (MHFGQ_27360) - 2646862..2647053 (-) 192 WP_170066261.1 DUF1858 domain-containing protein -
  MHFGQ_RS13740 (MHFGQ_27370) - 2647148..2647456 (-) 309 WP_106005300.1 MTH1187 family thiamine-binding protein -
  MHFGQ_RS13750 (MHFGQ_27390) - 2647671..2648378 (-) 708 WP_106005301.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48571.32 Da        Isoelectric Point: 6.8333

>NTDB_id=890875 MHFGQ_RS13720 WP_245907827.1 2642118..2643476(-) (radA) [Moorella humiferrea strain 64-FGQ]
MAKIKERFICQVCGYETWSYLGRCPSCGSWNSLVVEVVAGEMVKGRGRKEAVPSLLTEIADIKGERLVSMLGEWDRVLGG
GLVPGSLILVGGAPGIGKSTLLLQVAHSLAERYGKILYISGEESPEQVKLRAQRLKALKGPIYFLAETDVEGVLAGIEGL
DPVVAIIDSIQTMYLPAIQAAPGSVSQVRECAARFQRLAKKGGTAVILVGHVTKEGFLAGPRVLEHLVDTVLYLEGERYQ
AYRILRAAKNRFGSTNEIGIFEMDARGLKEVANPSETLLAERPVGVAGSSVAACMEGTRPLLLEIQALVSPTSFGNPRRL
TTGVDYNRALLLTAVLEKRAGLNLAGHDVYLNVAGGITINEPAADLAICLAIASGFKDRPVAQETLVLGEVGLAGEVRAV
SQLERRIEEAIRLGFKRFIIPAGNKSSLFSQNDCEFYSVRSVIEALEVGLEI

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=890875 MHFGQ_RS13720 WP_245907827.1 2642118..2643476(-) (radA) [Moorella humiferrea strain 64-FGQ]
TTGGCCAAAATAAAGGAGCGTTTTATCTGCCAGGTATGCGGTTACGAAACATGGAGCTACCTGGGCCGCTGCCCTTCCTG
CGGCAGTTGGAATAGTTTGGTGGTAGAAGTTGTAGCGGGAGAAATGGTGAAGGGAAGAGGCAGGAAGGAGGCAGTCCCCT
CCCTTTTGACGGAAATCGCCGATATTAAGGGTGAGCGTCTGGTCAGTATGCTGGGGGAATGGGACCGGGTCCTCGGGGGT
GGACTGGTTCCGGGCTCTTTGATCCTCGTCGGCGGCGCACCGGGCATCGGCAAATCCACCCTTCTTTTACAGGTAGCCCA
CAGCCTCGCCGAAAGGTACGGCAAAATCCTTTATATTTCAGGGGAAGAATCCCCAGAGCAGGTAAAACTCCGGGCCCAGC
GACTGAAGGCATTGAAGGGGCCGATATATTTCCTGGCCGAAACGGATGTCGAAGGTGTACTGGCCGGAATTGAAGGACTG
GACCCGGTAGTAGCCATCATTGATTCAATTCAAACAATGTACCTTCCGGCCATCCAGGCGGCACCGGGTAGCGTTTCCCA
GGTCCGGGAGTGTGCGGCGCGCTTTCAGCGCCTGGCCAAAAAAGGAGGCACGGCCGTCATCCTCGTCGGCCATGTTACCA
AAGAAGGTTTTCTGGCTGGCCCGCGTGTCCTTGAACACTTGGTGGATACCGTCCTCTACCTTGAAGGAGAACGCTACCAG
GCCTACCGTATCCTCCGGGCGGCTAAAAATCGCTTCGGTTCGACAAATGAAATCGGCATATTTGAAATGGATGCCCGGGG
GCTGAAAGAGGTGGCCAATCCTTCGGAAACCCTTTTAGCCGAACGGCCGGTCGGCGTTGCCGGCTCCAGTGTGGCGGCCT
GCATGGAAGGAACGCGGCCTCTGCTGCTGGAAATCCAGGCCCTGGTAAGCCCGACATCCTTCGGCAACCCCCGGCGGCTG
ACTACCGGCGTCGATTATAACCGTGCCCTGCTCCTTACGGCGGTCCTGGAAAAGCGGGCGGGCCTAAATTTGGCTGGCCA
CGACGTCTATCTCAATGTCGCCGGGGGCATTACTATAAATGAACCGGCCGCCGACCTGGCTATCTGTCTGGCCATAGCTT
CCGGTTTTAAAGACAGACCTGTAGCCCAAGAAACATTAGTTTTGGGGGAGGTGGGTCTGGCTGGGGAAGTCCGGGCCGTA
AGCCAGCTGGAGAGACGTATCGAAGAAGCCATCCGCCTGGGCTTTAAACGCTTTATAATACCCGCGGGAAATAAAAGCAG
CCTTTTTTCCCAGAATGATTGCGAATTTTATAGCGTGCGGTCGGTGATTGAGGCCTTAGAAGTCGGCCTGGAAATTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-216)

Subunit ChlI of Mg-chelatase (ChlI)

(340-424)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T0AS51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

55.044

100

0.555
  radA Streptococcus mitis SK321

53.437

99.779

0.533
  radA Streptococcus pneumoniae Rx1

53.437

99.779

0.533
  radA Streptococcus pneumoniae D39

53.437

99.779

0.533
  radA Streptococcus pneumoniae R6

53.437

99.779

0.533
  radA Streptococcus pneumoniae TIGR4

53.437

99.779

0.533
  radA Streptococcus mitis NCTC 12261

53.215

99.779

0.531