Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RVY74_RS20745 Genome accession   NZ_CP136372
Coordinates   4023697..4024740 (-) Length   347 a.a.
NCBI ID   WP_201038877.1    Uniprot ID   -
Organism   Bacillus mycoides strain PAMC 29434     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4018697..4029740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RVY74_RS20710 (RVY74_20710) - 4019851..4019976 (-) 126 WP_316281691.1 hypothetical protein -
  RVY74_RS20715 (RVY74_20715) - 4020147..4021316 (+) 1170 WP_316281692.1 tyrosine-type recombinase/integrase -
  RVY74_RS20720 (RVY74_20720) - 4021458..4021637 (-) 180 Protein_4025 Tfp pilus assembly protein FimT/FimU -
  RVY74_RS20725 (RVY74_20725) - 4021621..4021917 (-) 297 WP_002088621.1 hypothetical protein -
  RVY74_RS20730 (RVY74_20730) comGD 4021910..4022365 (-) 456 WP_002088622.1 competence type IV pilus minor pilin ComGD -
  RVY74_RS20735 (RVY74_20735) comGC 4022362..4022661 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  RVY74_RS20740 (RVY74_20740) comGB 4022673..4023710 (-) 1038 WP_002138336.1 competence type IV pilus assembly protein ComGB -
  RVY74_RS20745 (RVY74_20745) comGA 4023697..4024740 (-) 1044 WP_201038877.1 competence type IV pilus ATPase ComGA Machinery gene
  RVY74_RS20750 (RVY74_20750) - 4024946..4025641 (+) 696 WP_201038876.1 metalloregulator ArsR/SmtB family transcription factor -
  RVY74_RS20755 (RVY74_20755) - 4025919..4026161 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  RVY74_RS20760 (RVY74_20760) - 4026269..4027663 (+) 1395 WP_002138338.1 L-cystine transporter -
  RVY74_RS20765 (RVY74_20765) - 4027743..4028135 (-) 393 Protein_4034 hypothetical protein -
  RVY74_RS20770 (RVY74_20770) - 4028223..4028438 (-) 216 WP_002034026.1 DUF3912 family protein -
  RVY74_RS20775 (RVY74_20775) - 4028691..4029002 (+) 312 WP_201064368.1 hypothetical protein -
  RVY74_RS20780 (RVY74_20780) - 4029039..4029527 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39065.48 Da        Isoelectric Point: 9.3369

>NTDB_id=890411 RVY74_RS20745 WP_201038877.1 4023697..4024740(-) (comGA) [Bacillus mycoides strain PAMC 29434]
MNSVELFANMIMKEACGVQASDLHIVPRQKDVAIQLRIGKDLITKRCIGKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASAQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=890411 RVY74_RS20745 WP_201038877.1 4023697..4024740(-) (comGA) [Bacillus mycoides strain PAMC 29434]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTGGGGTGCAAGCATCAGACTTACATATTGTGCC
CAGGCAAAAGGATGTGGCGATTCAATTACGTATAGGGAAAGATTTAATTACGAAACGGTGTATTGGAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAGCCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGCTCAGCCGTTGTCTCACCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGATTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

42.955

83.862

0.36