Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   RVY74_RS05750 Genome accession   NZ_CP136372
Coordinates   1130266..1133781 (+) Length   1171 a.a.
NCBI ID   WP_201066371.1    Uniprot ID   -
Organism   Bacillus mycoides strain PAMC 29434     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1125266..1138781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RVY74_RS05710 (RVY74_05710) - 1125940..1126434 (-) 495 WP_002011284.1 DUF6584 family protein -
  RVY74_RS05715 (RVY74_05715) cspA 1126710..1126913 (+) 204 WP_002011282.1 RNA chaperone/antiterminator CspA -
  RVY74_RS05720 (RVY74_05720) - 1126940..1127201 (+) 262 Protein_1027 hypothetical protein -
  RVY74_RS05725 (RVY74_05725) - 1127252..1127437 (-) 186 WP_002011281.1 hypothetical protein -
  RVY74_RS05730 (RVY74_05730) - 1127731..1127817 (+) 87 Protein_1029 RNA-guided endonuclease TnpB family protein -
  RVY74_RS05735 (RVY74_05735) - 1127867..1128448 (+) 582 WP_002011279.1 competence protein ComK -
  RVY74_RS05740 (RVY74_05740) - 1128783..1129376 (+) 594 WP_002011278.1 TVP38/TMEM64 family protein -
  RVY74_RS05745 (RVY74_05745) lepB 1129585..1130148 (+) 564 WP_002011277.1 signal peptidase I -
  RVY74_RS05750 (RVY74_05750) addB 1130266..1133781 (+) 3516 WP_201066371.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  RVY74_RS05755 (RVY74_05755) addA 1133778..1137503 (+) 3726 WP_316281905.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  RVY74_RS05760 (RVY74_05760) - 1137527..1137805 (+) 279 WP_002011271.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  RVY74_RS05765 (RVY74_05765) - 1138021..1138728 (+) 708 WP_201051505.1 DNA alkylation repair protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134311.28 Da        Isoelectric Point: 5.1194

>NTDB_id=890388 RVY74_RS05750 WP_201066371.1 1130266..1133781(+) (addB) [Bacillus mycoides strain PAMC 29434]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLIEKLSDSEYVKGAEIYIDGFHSFSPQELEIVRGLMKLGTRVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDVAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRVRAEENGDFLFATDHEQVWEEVMNLLDTFVEMLGEEK
MSLSMFTEVMSTGLEALQFANIPPSLDQVLVANIDHSRLSDIKATFIIGVNEGVIPAAPMDEGMLSDEERNVLTAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTDVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADKLLRENRTWADLSIKECEHLSALVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGSTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=890388 RVY74_RS05750 WP_201066371.1 1130266..1133781(+) (addB) [Bacillus mycoides strain PAMC 29434]
ATGTCACTTCGATTTGTGATTGGTAGAGCTGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGGA
GCGAAGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCGCGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCCGGTGTACATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTTCAAAAAGCAGCGGAGCAAAACGGTTTTTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTATA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGTAGTGCAGAGCAAAAGCTGCTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTCGAACGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
CTTACAATTATTAATTGAGAAGCTTTCAGACTCTGAGTATGTAAAAGGCGCGGAAATTTATATAGATGGATTCCATTCAT
TTTCCCCTCAAGAGCTAGAAATTGTTAGAGGGTTAATGAAATTAGGAACTAGAGTAACAATAACATTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCGCTGATGGAACAGCCGCGTTTTCATTCGCAAGCATTAGCACATT
TAGAAACGCACTACGAAGCACGTCCAAATGAAAAATTTCATGGTGAAGCGAGTGTGACAATCAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTAGCCCGTGAAATTAGAAGACTTGTGGCGGATGAAGACTATCGTTACCGAGATGTCGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGACGTAATGCGAACGTTATTTACGGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCAATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCATTAGAAATTATTAGTGGGAATTGGCGTTAT
GATGCAGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGATGTAAGAAAAGAAGCGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGTT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAAAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGAATTACGTGTTCGTGCAGAAGAAAATGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAATCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCCCTTTCAATGTTTACGGAGGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCGAACATTCCGCCGTCACTAGA
TCAAGTGTTAGTTGCTAACATTGATCATTCTAGGTTATCAGATATTAAAGCGACGTTCATTATTGGAGTAAATGAAGGAG
TGATTCCGGCAGCGCCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAAATGTTCTCACCGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGAGCATCTGAGAAGTTATA
CATTTCCTGTCCGCTTGCGGATGAAGAAGGAAAGACGTTACTTGCTTCTAGTTTTATTAAAAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCGTTTATTACGAATGATGTAAATGACTTATCGCGTTCGGAACAAATCTCATATGTAGCAACGCCA
GAAGTAACGTTGTCTTATGTCATGCAGCAACTGCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGTGTGTTATCAAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGATGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGCTGTGCGTACGCTCATTTTGCTCAGCACGGTTTATCGCTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAAGTTATTACGTGAAAATCGGACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAACACCTTTCTGCTTTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCGAACCGTCATTTCTATTTAAAGCAAAAACTACAACAAATCATTTTCCGTACGTCACTTATTCTTCGTGAACA
TGCGAAGTCGAGTGGATTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGCGGGACTGGATCTCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAGAGTGGT
ACGTTCCTTCGTATTATTGATTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGCAGTACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCGATTGTTGAGATGAAAGGCGATGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTGCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAATCACTTGAAGATAA
CCAATTCCGTACGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(302-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.148

100

0.493