Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   RW107_RS12860 Genome accession   NZ_CP136257
Coordinates   2521220..2521987 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain A9     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2516220..2526987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RW107_RS12840 (RW107_12840) ppsR 2516394..2517206 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  RW107_RS12845 (RW107_12845) ccpN 2517232..2517870 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  RW107_RS12850 (RW107_12850) glyS 2518003..2520042 (-) 2040 WP_128441734.1 glycine--tRNA ligase subunit beta -
  RW107_RS12855 (RW107_12855) glyQ 2520035..2520922 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  RW107_RS12860 (RW107_12860) recO 2521220..2521987 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  RW107_RS12865 (RW107_12865) yqzL 2522024..2522167 (-) 144 WP_003230053.1 YqzL family protein -
  RW107_RS12870 (RW107_12870) era 2522315..2523220 (-) 906 WP_003230051.1 GTPase Era -
  RW107_RS12875 (RW107_12875) - 2523201..2523611 (-) 411 WP_003230049.1 cytidine deaminase -
  RW107_RS12880 (RW107_12880) unpK 2523730..2524101 (-) 372 WP_046160604.1 diacylglycerol kinase family protein -
  RW107_RS12885 (RW107_12885) ybeY 2524082..2524555 (-) 474 WP_032722131.1 rRNA maturation RNase YbeY -
  RW107_RS12890 (RW107_12890) pgpH 2524556..2526691 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=889896 RW107_RS12860 WP_014477369.1 2521220..2521987(-) (recO) [Bacillus subtilis strain A9]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=889896 RW107_RS12860 WP_014477369.1 2521220..2521987(-) (recO) [Bacillus subtilis strain A9]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGTCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGTATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996