Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   AAEO47_RS22070 Genome accession   NZ_CP151543
Coordinates   4785371..4785967 (+) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain HN232     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4780371..4790967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEO47_RS22060 (AAEO47_22060) - 4781654..4784122 (-) 2469 WP_003114576.1 penicillin-binding protein 1A -
  AAEO47_RS22065 (AAEO47_22065) pilM 4784307..4785371 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  AAEO47_RS22070 (AAEO47_22070) pilN 4785371..4785967 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  AAEO47_RS22075 (AAEO47_22075) pilO 4785964..4786587 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAEO47_RS22080 (AAEO47_22080) comL/pilP 4786584..4787108 (+) 525 WP_257319086.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  AAEO47_RS22085 (AAEO47_22085) pilQ 4787162..4789306 (+) 2145 WP_012614629.1 type 4a pilus secretin PilQ Machinery gene
  AAEO47_RS22090 (AAEO47_22090) aroK 4789318..4789836 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=889030 AAEO47_RS22070 WP_003095839.1 4785371..4785967(+) (pilN) [Pseudomonas aeruginosa strain HN232]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=889030 AAEO47_RS22070 WP_003095839.1 4785371..4785967(+) (pilN) [Pseudomonas aeruginosa strain HN232]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369