Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   RWA16_RS01220 Genome accession   NZ_CP136128
Coordinates   258828..260627 (-) Length   599 a.a.
NCBI ID   WP_087911393.1    Uniprot ID   A0AAN1EXR0
Organism   Lacticaseibacillus casei strain LMG 24099     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 253828..265627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RWA16_RS01195 (RWA16_01195) - 255093..255437 (+) 345 WP_025013473.1 hypothetical protein -
  RWA16_RS01200 (RWA16_01200) - 255511..256230 (+) 720 WP_039639686.1 metal ABC transporter ATP-binding protein -
  RWA16_RS01205 (RWA16_01205) - 256227..257024 (+) 798 WP_010489770.1 metal ABC transporter permease -
  RWA16_RS01210 (RWA16_01210) - 257105..257953 (-) 849 WP_087911391.1 metallophosphoesterase family protein -
  RWA16_RS01215 (RWA16_01215) - 257950..258831 (-) 882 WP_087911392.1 NAD(P)-dependent oxidoreductase -
  RWA16_RS01220 (RWA16_01220) pepF 258828..260627 (-) 1800 WP_087911393.1 oligoendopeptidase F Regulator
  RWA16_RS01225 (RWA16_01225) - 260756..261850 (+) 1095 WP_087911394.1 LCP family protein -
  RWA16_RS01230 (RWA16_01230) brnQ 262029..263408 (-) 1380 WP_087913212.1 branched-chain amino acid transport system II carrier protein -
  RWA16_RS01235 (RWA16_01235) - 264084..264767 (-) 684 WP_047106501.1 hydrolase -

Sequence

Protein


Download         Length: 599 a.a.        Molecular weight: 67294.34 Da        Isoelectric Point: 5.5402

>NTDB_id=888736 RWA16_RS01220 WP_087911393.1 258828..260627(-) (pepF) [Lacticaseibacillus casei strain LMG 24099]
MQHLPVREDVPTKLTWDLTTIYPNDAAWTADFEAVREQAKEAAALKGTLGNSAAALINGIKAVLAVFRRFEKVYVYSSLK
SDQDTGNATYQAMNAKAESLGAELASQLAFLDPEILAIPADKLTAWRDTESLKPFGHFIDAITVNRQHVLTTAAEALIAS
AGDALNASHATFNVLDNSDLQFGFVENEDGETVQLSNGLYGQLIRSTQRKLRKEAFETLLRAYESMKNTFAQTLSGQVKA
HNFNAKAHHYVNARAAAMASNHIPESVYTTLIDQVNTHLPLLHRYVALRKKVLGVDQLHMYDIYTPLTGKPPLTFTLDQA
KTEALKALAPLGDDYLSHVREIFDNRYIDVVENKGKRSGAYSGGSYDTNPFILLNWHDAVDELYTLIHETGHSVHSWYTR
HNQPYVYGDYPIFVAEIASTTNENLLTDYFLTHTDDPKVRAYILNYYLDGFKGTVFRQTQFAEFEHWIHQQDQQGEPLTA
TSMSTYYADLNARYYGPDVTRDPEIAFEWARIPHFYYNYYVYQYATGFAAASTLAAGISSGEPNAASRYLDYLKSGSSQY
AIDTMKTAGVDMTKPDYLEAAFSMFEKRLTELEELLQKG

Nucleotide


Download         Length: 1800 bp        

>NTDB_id=888736 RWA16_RS01220 WP_087911393.1 258828..260627(-) (pepF) [Lacticaseibacillus casei strain LMG 24099]
ATGCAACATTTACCGGTACGTGAAGATGTCCCAACCAAGCTGACATGGGATCTAACCACCATTTATCCAAATGATGCGGC
GTGGACAGCCGACTTTGAAGCTGTCCGCGAACAGGCCAAAGAGGCGGCCGCGCTCAAGGGAACGTTAGGCAATTCAGCCG
CTGCGCTCATCAACGGCATCAAGGCGGTTTTAGCGGTCTTTCGACGGTTTGAGAAAGTCTATGTTTATTCAAGTCTGAAA
AGCGACCAGGATACTGGCAACGCCACTTATCAGGCGATGAATGCTAAGGCCGAAAGTCTCGGCGCCGAGCTGGCCAGTCA
GTTAGCCTTTTTGGATCCGGAGATTCTGGCAATTCCCGCGGACAAACTGACAGCATGGCGGGACACTGAAAGTCTCAAAC
CGTTTGGGCATTTTATTGACGCGATCACCGTCAATCGCCAACATGTGCTGACAACCGCAGCCGAGGCTCTGATTGCCAGT
GCCGGTGACGCGCTGAACGCCAGTCATGCCACTTTCAACGTCCTAGACAACTCCGACCTGCAATTCGGTTTTGTCGAAAA
TGAAGACGGTGAAACAGTGCAGCTCAGTAACGGCTTGTACGGCCAGTTGATCCGCTCAACCCAGCGAAAATTGCGCAAAG
AAGCCTTTGAAACCTTACTGCGTGCTTATGAAAGCATGAAGAACACGTTTGCCCAAACCCTGAGCGGCCAGGTCAAGGCG
CACAATTTCAATGCCAAAGCCCATCATTACGTTAATGCCCGTGCGGCGGCAATGGCAAGCAACCACATTCCGGAAAGTGT
TTATACGACCCTTATCGACCAGGTCAACACCCATTTGCCCCTTCTGCATCGCTATGTCGCATTGCGGAAAAAAGTCCTGG
GCGTCGATCAATTGCACATGTATGACATCTACACCCCGTTGACCGGCAAGCCGCCATTGACCTTCACGCTTGACCAAGCA
AAAACTGAAGCATTGAAAGCTTTGGCGCCGCTTGGTGATGATTATCTCAGCCATGTCCGGGAAATCTTTGACAACCGCTA
CATTGATGTCGTCGAAAATAAAGGCAAACGTTCCGGCGCTTATTCAGGTGGGTCTTACGATACGAATCCGTTTATTTTGC
TGAACTGGCATGACGCGGTGGACGAACTTTATACTTTGATTCACGAAACCGGCCACAGCGTTCACAGCTGGTACACGCGT
CACAACCAGCCTTATGTGTATGGCGATTACCCGATTTTTGTTGCCGAAATTGCGTCAACGACCAACGAAAATCTGCTCAC
CGATTACTTCCTGACCCACACGGATGACCCCAAGGTACGCGCCTACATCCTGAATTACTATCTCGATGGCTTTAAAGGTA
CCGTCTTCCGCCAAACGCAATTTGCCGAATTTGAACACTGGATTCATCAGCAGGACCAACAAGGCGAGCCACTCACGGCA
ACCAGTATGTCAACATACTACGCCGATCTCAATGCCCGGTATTATGGACCGGATGTTACCCGCGATCCCGAAATTGCCTT
CGAATGGGCACGAATCCCGCATTTCTATTACAATTATTATGTCTATCAGTATGCAACCGGTTTTGCCGCAGCATCAACGC
TAGCTGCCGGCATTAGCAGTGGCGAACCCAACGCCGCGTCCCGCTACTTGGACTATTTAAAGTCAGGTAGCTCGCAATAC
GCCATCGATACCATGAAAACCGCTGGTGTCGACATGACCAAACCGGACTATCTGGAAGCGGCATTTTCAATGTTTGAAAA
ACGGTTAACGGAATTGGAAGAACTTTTACAGAAAGGATGA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(203-582)

Oligopeptidase F (Peptidase_M3_N)

(114-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

52.694

99.165

0.523