Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RP299_RS06300 Genome accession   NZ_CP136013
Coordinates   1225645..1227024 (-) Length   459 a.a.
NCBI ID   WP_004896725.1    Uniprot ID   Q74L28
Organism   Lactobacillus johnsonii strain N5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1220645..1232024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP299_RS06280 (RP299_06280) rlmB 1221384..1222139 (-) 756 WP_004895769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  RP299_RS06285 (RP299_06285) - 1222126..1222569 (-) 444 WP_004895772.1 Mini-ribonuclease 3 -
  RP299_RS06290 (RP299_06290) cysS 1222562..1223974 (-) 1413 WP_004896726.1 cysteine--tRNA ligase -
  RP299_RS06295 (RP299_06295) gltX 1224064..1225563 (-) 1500 WP_004895777.1 glutamate--tRNA ligase -
  RP299_RS06300 (RP299_06300) radA 1225645..1227024 (-) 1380 WP_004896725.1 DNA repair protein RadA Machinery gene
  RP299_RS06305 (RP299_06305) - 1227024..1227575 (-) 552 WP_014567124.1 hypothetical protein -
  RP299_RS06310 (RP299_06310) - 1227693..1228145 (-) 453 WP_390882885.1 LCP family protein -
  RP299_RS06315 (RP299_06315) - 1228164..1228544 (-) 381 WP_232970587.1 hypothetical protein -
  RP299_RS06320 (RP299_06320) pepC 1228741..1230090 (+) 1350 WP_014567122.1 aminopeptidase C -
  RP299_RS06325 (RP299_06325) - 1230374..1231378 (+) 1005 WP_315943528.1 sugar transferase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50118.70 Da        Isoelectric Point: 7.6366

>NTDB_id=888238 RP299_RS06300 WP_004896725.1 1225645..1227024(-) (radA) [Lactobacillus johnsonii strain N5]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEIKKVSTKATASRLMTKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEHSVLYVSGEESASQIKMRADRLGVSNSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAIFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVNQIEARVKEAAKVGFKRIFIPKNNLNQELKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=888238 RP299_RS06300 WP_004896725.1 1225645..1227024(-) (radA) [Lactobacillus johnsonii strain N5]
ATGGCAAAAGTTAAAACTCGTTATAAGTGTCGTAATTGTGGATATATTTCTGCTTCCTATCTTGGAAGATGTCCCAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGACACAGGAAATTAAAAAAGTATCAACTAAGGCAACTGCAAGTCGGTTGATGA
CTAAAATTGGAAATAATGATCCAGTAAAGTTAACTGAAGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGGATTGTTCCAGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACCTTAATGCTTCAAATTACTGGCGCTTTAGCTAAAGAACATAGCGTCTTATATGTTTCAGGAGAAGAGTCCGCAAGTC
AAATAAAGATGCGGGCAGATCGATTAGGAGTAAGCAATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAGAATATT
CGTGACCAGATTGATGAAATTAAGCCAGATTTTTTGGTAATTGATTCTATTCAAACAATGAATGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAACTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGCCATGTAACTAAAGAAGGAGCAATTGCTGGGCCTAAGATTATGGAACATATGGTTGATACCGTTCTTTAC
TTTGAAGGAGATGGGCACCACTCATATCGAATTTTAAGATCAGTAAAGAATCGTTTTGGTGCAGCTAATGAAATTGGAAT
GTTTGAGATGAAAAATGAAGGGTTAGCAGAAGTAAGTAATCCTTCAGCTATTTTCTTAGACGAACGCTTACCTAATTCGA
CTGGTTCCTCAGTTGTTGTTTCACTTGAAGGAACGCGGCCTCTTCTTGCGGATATCCAAGCTTTAGTCACCCCAACAGCA
TTCGGATATGCAAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGGCAGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAATCAAGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCAATTGATCTTG
CAATTTGCATGGCAGTTGCTTCAAGTTATAAAAATAAAGAAATTTCTTCGACTGATTGCTTTGTAGGTGAAGTTGGCTTG
ACTGGTGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTTAAAGAGGCCGCAAAAGTAGGCTTTAAGCGAATTTTTAT
TCCTAAAAACAACTTAAACCAGGAGCTTAAGAATAACTCTGAGATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q74L28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.262

99.782

0.601
  radA Streptococcus pneumoniae Rx1

60.262

99.782

0.601
  radA Streptococcus pneumoniae D39

60.262

99.782

0.601
  radA Streptococcus pneumoniae R6

60.262

99.782

0.601
  radA Streptococcus pneumoniae TIGR4

60.262

99.782

0.601
  radA Streptococcus mitis SK321

60.262

99.782

0.601
  radA Bacillus subtilis subsp. subtilis str. 168

56.14

99.346

0.558