Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   RUW01_RS06150 Genome accession   NZ_CP136012
Coordinates   1200850..1202658 (+) Length   602 a.a.
NCBI ID   WP_315943412.1    Uniprot ID   -
Organism   Lactobacillus johnsonii strain N7     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1180635..1209928 1200850..1202658 within 0


Gene organization within MGE regions


Location: 1180635..1209928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RUW01_RS06045 - 1180635..1181381 (-) 747 WP_023599967.1 ABC transporter ATP-binding protein -
  RUW01_RS06050 - 1181621..1183069 (+) 1449 WP_069168697.1 APC family permease -
  RUW01_RS06055 hflX 1183850..1185130 (+) 1281 WP_004897990.1 GTPase HflX -
  RUW01_RS06060 - 1185395..1186617 (+) 1223 Protein_1134 IS110 family transposase -
  RUW01_RS06065 - 1186833..1188041 (-) 1209 WP_315943406.1 L,D-transpeptidase/peptidoglycan binding protein -
  RUW01_RS06070 - 1188145..1189410 (-) 1266 WP_127836021.1 C40 family peptidase -
  RUW01_RS06075 - 1189604..1190161 (-) 558 WP_127836022.1 guanylate kinase -
  RUW01_RS06080 - 1190252..1190545 (+) 294 WP_315943407.1 DUF2187 domain-containing protein -
  RUW01_RS06085 - 1190595..1192121 (+) 1527 WP_315943408.1 O-antigen ligase family protein -
  RUW01_RS06090 - 1192192..1192818 (-) 627 WP_086875182.1 LVIS_2131 family protein -
  RUW01_RS06095 nrdI 1192960..1193421 (+) 462 WP_004897997.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  RUW01_RS06100 - 1193414..1194355 (+) 942 WP_315943409.1 ribonucleotide-diphosphate reductase subunit beta -
  RUW01_RS06105 - 1194380..1194865 (-) 486 WP_004897999.1 hypothetical protein -
  RUW01_RS06110 - 1194951..1195292 (-) 342 WP_004898000.1 type II toxin-antitoxin system PemK/MazF family toxin -
  RUW01_RS06115 mazE 1195279..1195530 (-) 252 WP_004898001.1 type II toxin-antitoxin system PemI/MazE family antitoxin -
  RUW01_RS06120 - 1195762..1196232 (-) 471 WP_127836026.1 threonine/serine exporter family protein -
  RUW01_RS06125 - 1196232..1197014 (-) 783 WP_315943410.1 threonine/serine exporter family protein -
  RUW01_RS06130 - 1197031..1198578 (-) 1548 WP_014567846.1 ABC-F family ATP-binding cassette domain-containing protein -
  RUW01_RS06135 - 1198578..1198961 (-) 384 WP_014567847.1 hypothetical protein -
  RUW01_RS06140 - 1199185..1199457 (+) 273 WP_014567848.1 hypothetical protein -
  RUW01_RS06145 - 1199562..1200686 (-) 1125 WP_315943411.1 LCP family protein -
  RUW01_RS06150 pepF 1200850..1202658 (+) 1809 WP_315943412.1 oligoendopeptidase F Regulator
  RUW01_RS06155 - 1202728..1204239 (-) 1512 WP_004898009.1 L-lactate permease -
  RUW01_RS06160 - 1204360..1205151 (-) 792 WP_011162511.1 metal ABC transporter permease -
  RUW01_RS06165 - 1205151..1205810 (-) 660 WP_315943413.1 ATP-binding cassette domain-containing protein -
  RUW01_RS06170 - 1205849..1206748 (-) 900 WP_004898012.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  RUW01_RS06175 - 1207049..1207246 (-) 198 Protein_1157 ArsR/SmtB family transcription factor -
  RUW01_RS06180 - 1207421..1208643 (-) 1223 Protein_1158 IS110 family transposase -
  RUW01_RS06185 galE 1208936..1209928 (-) 993 WP_069168699.1 UDP-glucose 4-epimerase GalE -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 68607.26 Da        Isoelectric Point: 4.5875

>NTDB_id=888219 RUW01_RS06150 WP_315943412.1 1200850..1202658(+) (pepF) [Lactobacillus johnsonii strain N7]
MAIPTRNEVSDDLKWDLSRVFKDDQEWEQEYNQVATEIKNLGRFKGNLAKSGQDLYEGITEILSVNRRLEKVYVYATMSS
DVDTSNTHYLGLVAKAQSLANQMSAAISFVDPEILSIPEETLAKFMQDEPRLKEYQHRLEQITKKRPHTLPASEEKIIAA
AGDAIGTSANTFNVLTNSDMEYGYVQDEDGEMVQLSDGLYSLLIQSQDRNVRKNAFDVMYASYGQFENSLASTLSGEVKA
HNFNAQVHKYNSAREAALSENGVPTAVYDTLVKEVNSHLNLLHRYVSLRKKILGLSDLQMYDMYVPLTGEPSLSYNFEEA
KNEARRALAPLGKDYLEHVDYIFNNRVIDVVESQNKVTGAYSGGSYDTDPYELLNWEDNLDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDKITDPKTRAFVLNHYLDSFKGTLFRQTQFAEFEQFIHEADANGQPLTA
DVLDEFYGNLNQRYYGDSVEPGGEIAKEWARIPHFYYNFYVYQYATGFAAATALANNVVHGTDEERTAYINFLKAGSSDY
PTEIMKRAGVDMTKADYLRDAFNTFEKRLNEFEKIVNELKTI

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=888219 RUW01_RS06150 WP_315943412.1 1200850..1202658(+) (pepF) [Lactobacillus johnsonii strain N7]
ATGGCTATTCCAACAAGAAATGAAGTATCTGATGATCTCAAATGGGATCTTAGTCGTGTTTTTAAAGACGATCAGGAATG
GGAACAAGAATATAATCAGGTCGCAACCGAAATTAAAAATTTAGGTAGATTTAAGGGAAATCTTGCTAAATCTGGTCAAG
ATTTATATGAAGGAATTACAGAAATTTTATCTGTAAATCGTCGTTTAGAAAAAGTGTACGTTTATGCTACTATGTCAAGC
GACGTTGATACTAGTAATACTCATTATCTAGGCCTCGTTGCTAAAGCTCAAAGCTTAGCTAACCAGATGAGCGCCGCTAT
CTCTTTTGTCGATCCTGAAATTTTATCTATCCCAGAAGAAACACTTGCTAAATTTATGCAAGATGAGCCCCGTTTAAAAG
AGTATCAACATCGACTTGAACAAATTACTAAGAAACGTCCTCATACTTTACCAGCAAGTGAAGAAAAAATTATTGCCGCT
GCTGGAGATGCAATAGGAACTTCCGCTAATACTTTCAATGTTTTAACTAATTCTGATATGGAATACGGCTATGTTCAAGA
TGAAGACGGAGAGATGGTCCAATTATCTGATGGCTTATATTCACTTCTAATTCAGTCACAAGATAGAAATGTTCGCAAAA
ATGCATTTGATGTTATGTATGCAAGCTATGGCCAGTTTGAAAATAGTCTCGCTTCAACTTTATCAGGTGAAGTAAAAGCC
CACAATTTTAATGCTCAAGTACATAAATATAATTCAGCTCGAGAGGCTGCTTTAAGTGAAAATGGCGTACCAACAGCTGT
TTACGATACATTGGTTAAAGAGGTAAATAGTCACCTCAACTTACTTCATCGCTACGTTAGTTTAAGAAAAAAGATTTTAG
GTCTTTCTGATTTACAAATGTATGATATGTATGTTCCATTAACAGGTGAGCCCAGTCTTTCTTATAACTTTGAAGAAGCA
AAAAATGAAGCTAGACGAGCCTTAGCGCCATTAGGAAAAGATTACCTCGAACATGTTGATTACATCTTTAATAATCGTGT
AATTGATGTGGTTGAAAGCCAAAATAAGGTAACAGGCGCTTATTCTGGTGGTTCCTATGATACTGATCCATATGAATTAT
TAAATTGGGAGGATAATCTTGATTCTCTATATACTCTAGTTCATGAAACTGGACACTCAGTGCATTCCTGGTATACAAGA
AATACTCAACCATATGTATATGGAGACTACCCAATTTTTGTTGCTGAAATTGCATCAACAACTAATGAAAATATTTTAAC
TGAATACTTCTTAGATAAAATTACCGATCCTAAAACACGCGCATTTGTTTTGAATCACTATCTTGATTCATTTAAAGGTA
CACTTTTCCGTCAAACACAATTTGCAGAATTTGAGCAGTTTATTCATGAAGCTGATGCAAATGGTCAACCATTAACTGCC
GATGTTCTTGATGAATTTTATGGCAATTTAAACCAAAGATATTACGGTGATAGTGTAGAACCGGGCGGAGAGATTGCTAA
AGAATGGGCCCGTATTCCTCACTTCTATTACAACTTCTATGTTTACCAATATGCAACAGGTTTTGCTGCTGCTACAGCCC
TTGCAAATAATGTTGTTCATGGTACGGACGAAGAACGTACAGCTTATATTAATTTCTTAAAGGCTGGTTCTTCAGATTAT
CCAACAGAAATAATGAAACGTGCCGGTGTCGATATGACAAAGGCTGATTACTTAAGAGACGCATTTAATACTTTTGAAAA
ACGACTTAATGAGTTTGAAAAAATCGTTAATGAACTGAAAACAATCTAG

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(203-583)

Oligopeptidase F (Peptidase_M3_N)

(113-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.689

98.339

0.508