Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RO483_RS13865 Genome accession   NZ_CP135968
Coordinates   2987438..2988031 (-) Length   197 a.a.
NCBI ID   WP_054577258.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain S10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982438..2993031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RO483_RS13845 (RO483_13845) aroB 2983332..2984432 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  RO483_RS13850 (RO483_13850) aroK 2984465..2984983 (-) 519 WP_005381319.1 shikimate kinase AroK -
  RO483_RS13855 (RO483_13855) pilQ 2985171..2986907 (-) 1737 WP_315956623.1 type IV pilus secretin PilQ family protein Machinery gene
  RO483_RS13860 (RO483_13860) pilP 2986933..2987448 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  RO483_RS13865 (RO483_13865) pilO 2987438..2988031 (-) 594 WP_054577258.1 type 4a pilus biogenesis protein PilO Machinery gene
  RO483_RS13870 (RO483_13870) pilN 2988024..2988599 (-) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  RO483_RS13875 (RO483_13875) pilM 2988583..2989599 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  RO483_RS13880 (RO483_13880) - 2989744..2992305 (+) 2562 WP_025768081.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23043.59 Da        Isoelectric Point: 5.0948

>NTDB_id=887938 RO483_RS13865 WP_054577258.1 2987438..2988031(-) (pilO) [Vibrio alginolyticus strain S10]
MVDWQDLEIDEMAEWPLLPQIIVIALLIMVIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=887938 RO483_RS13865 WP_054577258.1 2987438..2988031(-) (pilO) [Vibrio alginolyticus strain S10]
GTGGTTGATTGGCAGGATCTCGAAATCGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTATAGTAATCGCGCTTCT
GATCATGGTGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACCCGTTACGAGTTATTGCTGCAGCAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
ACTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTCC
CTTTAAACATTGAGTTAACGGGTGACTTTCATGACATCGGCCATTTTTCTCAGGCGATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACTCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.368

96.447

0.843
  pilO Vibrio cholerae strain A1552

70.157

96.954

0.68