Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RS960_RS02525 Genome accession   NZ_CP135966
Coordinates   532445..533809 (+) Length   454 a.a.
NCBI ID   WP_317528876.1    Uniprot ID   -
Organism   Staphylococcus aureus strain ST5477     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 527445..538809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS960_RS02510 (RS960_02510) - 527923..528489 (+) 567 WP_262537363.1 UvrB/UvrC motif-containing protein -
  RS960_RS02515 (RS960_02515) - 528479..529486 (+) 1008 WP_262607889.1 protein arginine kinase -
  RS960_RS02520 (RS960_02520) - 529500..531956 (+) 2457 WP_000897132.1 ATP-dependent Clp protease ATP-binding subunit -
  RS960_RS02525 (RS960_02525) radA 532445..533809 (+) 1365 WP_317528876.1 DNA repair protein RadA Machinery gene
  RS960_RS02530 (RS960_02530) - 533834..534907 (+) 1074 WP_262537360.1 PIN/TRAM domain-containing protein -
  RS960_RS02535 (RS960_02535) gltX 535464..536918 (+) 1455 WP_001283787.1 glutamate--tRNA ligase -
  RS960_RS02540 (RS960_02540) cysE 537341..537988 (+) 648 WP_001805237.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49798.35 Da        Isoelectric Point: 6.8205

>NTDB_id=887866 RS960_RS02525 WP_317528876.1 532445..533809(+) (radA) [Staphylococcus aureus strain ST5477]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
CVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYLEGIQVIGVTTVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=887866 RS960_RS02525 WP_317528876.1 532445..533809(+) (radA) [Staphylococcus aureus strain ST5477]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTAAAAACCAAGGAATTAGCAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCGAGAGTGTTAACAGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTATTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCGTCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
TGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCTTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCATTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.231

100

0.67
  radA Streptococcus pneumoniae Rx1

61.607

98.678

0.608
  radA Streptococcus pneumoniae D39

61.607

98.678

0.608
  radA Streptococcus pneumoniae R6

61.607

98.678

0.608
  radA Streptococcus pneumoniae TIGR4

61.607

98.678

0.608
  radA Streptococcus mitis NCTC 12261

61.607

98.678

0.608
  radA Streptococcus mitis SK321

61.607

98.678

0.608