Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RS401_RS19915 Genome accession   NZ_CP135601
Coordinates   3849906..3850340 (-) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus sp. SI2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3844906..3855340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS401_RS19900 (RS401_19900) - 3845669..3846226 (+) 558 WP_001989622.1 PadR family transcriptional regulator -
  RS401_RS19905 (RS401_19905) metE 3847155..3849443 (+) 2289 WP_071736894.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  RS401_RS19910 (RS401_19910) comJ 3849480..3849869 (-) 390 WP_000424033.1 competence protein ComJ -
  RS401_RS19915 (RS401_19915) nucA/comI 3849906..3850340 (-) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  RS401_RS19920 (RS401_19920) - 3850577..3851326 (+) 750 WP_061657933.1 DUF3967 domain-containing protein -
  RS401_RS19925 (RS401_19925) kinB 3851380..3852654 (-) 1275 WP_000420390.1 sporulation sensor histidine kinase KinB -
  RS401_RS19930 (RS401_19930) - 3852964..3853164 (+) 201 WP_000929284.1 hypothetical protein -
  RS401_RS19935 (RS401_19935) - 3853287..3854450 (+) 1164 WP_000434570.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=887118 RS401_RS19915 WP_000811492.1 3849906..3850340(-) (nucA/comI) [Bacillus sp. SI2]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=887118 RS401_RS19915 WP_000811492.1 3849906..3850340(-) (nucA/comI) [Bacillus sp. SI2]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCGTACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556