Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RS401_RS00385 Genome accession   NZ_CP135601
Coordinates   68888..70264 (+) Length   458 a.a.
NCBI ID   WP_001085212.1    Uniprot ID   A0A2B3Y2M8
Organism   Bacillus sp. SI2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 63888..75264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS401_RS00365 (RS401_00365) ctsR 64081..64542 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  RS401_RS00370 (RS401_00370) - 64716..65264 (+) 549 WP_000128391.1 UvrB/UvrC motif-containing protein -
  RS401_RS00375 (RS401_00375) - 65269..66333 (+) 1065 WP_000050832.1 protein arginine kinase -
  RS401_RS00380 (RS401_00380) clpC 66356..68791 (+) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  RS401_RS00385 (RS401_00385) radA 68888..70264 (+) 1377 WP_001085212.1 DNA repair protein RadA Machinery gene
  RS401_RS00390 (RS401_00390) disA 70268..71341 (+) 1074 WP_000392168.1 DNA integrity scanning diadenylate cyclase DisA -
  RS401_RS00395 (RS401_00395) - 71502..72611 (+) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  RS401_RS00400 (RS401_00400) ispD 72628..73308 (+) 681 WP_000288292.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RS401_RS00405 (RS401_00405) ispF 73423..73899 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49858.44 Da        Isoelectric Point: 7.1421

>NTDB_id=887093 RS401_RS00385 WP_001085212.1 68888..70264(+) (radA) [Bacillus sp. SI2]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=887093 RS401_RS00385 WP_001085212.1 68888..70264(+) (radA) [Bacillus sp. SI2]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAA
CAGAAGTAACAAAACCAAGGCGCCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTCCAAGAATTT
AACCGTGTACTTGGTGGTGGGATTGTAGATGGATCCTTAGTACTTATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCGGATTCTTCATATGATGTACTATATATATCGGGTGAGGAATCAGCAAAACAGA
TTAAACTGCGTGCCGATCGTCTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
ACACACATTGAAGAGGTGAACCCAGCCTTTGTTGTTATTGACTCTATTCAAACAATACATTTACCTGAGGTAACATCAGC
GCCGGGAAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGCTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCAGAAGTATTAAACCCTTCTGAGATTTTCCTTGAGGAAAGACCAGTGGGGGTTGCGG
GATCAACAGTGGTTGCTTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTGTTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGACGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCTATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTCAGAGATAAATCTACAGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCGGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B3Y2M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629