Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RRL13_RS20160 Genome accession   NZ_CP135498
Coordinates   4263899..4265332 (+) Length   477 a.a.
NCBI ID   WP_182062678.1    Uniprot ID   -
Organism   Enterobacter cloacae strain isolateB     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4258899..4270332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRL13_RS20130 (RRL13_20130) - 4259466..4260443 (+) 978 WP_315686946.1 calcium/sodium antiporter -
  RRL13_RS20135 (RRL13_20135) kdsD 4260457..4261443 (+) 987 WP_182062670.1 arabinose-5-phosphate isomerase KdsD -
  RRL13_RS20140 (RRL13_20140) kdsC 4261458..4262024 (+) 567 WP_182063475.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  RRL13_RS20145 (RRL13_20145) lptC 4262021..4262596 (+) 576 WP_182062672.1 LPS export ABC transporter periplasmic protein LptC -
  RRL13_RS20150 (RRL13_20150) lptA 4262565..4263119 (+) 555 WP_182062674.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  RRL13_RS20155 (RRL13_20155) lptB 4263126..4263851 (+) 726 WP_182062676.1 LPS export ABC transporter ATP-binding protein -
  RRL13_RS20160 (RRL13_20160) rpoN/rpoN1 4263899..4265332 (+) 1434 WP_182062678.1 RNA polymerase factor sigma-54 Machinery gene
  RRL13_RS20165 (RRL13_20165) hpf 4265355..4265642 (+) 288 WP_182062680.1 ribosome hibernation promoting factor -
  RRL13_RS20170 (RRL13_20170) ptsN 4265725..4266216 (+) 492 WP_014071955.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RRL13_RS20175 (RRL13_20175) rapZ 4266262..4267116 (+) 855 WP_182062681.1 RNase adapter RapZ -
  RRL13_RS20180 (RRL13_20180) npr 4267113..4267385 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  RRL13_RS20185 (RRL13_20185) mtgA 4267423..4268148 (-) 726 WP_182062682.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  RRL13_RS20190 (RRL13_20190) elbB 4268145..4268798 (-) 654 WP_182063476.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54236.02 Da        Isoelectric Point: 4.3173

>NTDB_id=886896 RRL13_RS20160 WP_182062678.1 4263899..4265332(+) (rpoN/rpoN1) [Enterobacter cloacae strain isolateB]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQTQDTETLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDD
ILESIGDDEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWIEEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=886896 RRL13_RS20160 WP_182062678.1 4263899..4265332(+) (rpoN/rpoN1) [Enterobacter cloacae strain isolateB]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCACAGCTACAACAAGCGATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAACTCCAGCAAGCACTGGACAGCAACCCGTTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAAACGCAGGACACCGAAACGCTCGATACCGTTGATGCTCTCGAACAAAAAGAGATGCCGGAC
GAGCTGCCTTTGGATGCCAGTTGGGATGAAATTTACACCGCAGGAACCCCGTCCGGGACGCGTGCGGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGCGAAACTACCCAGTCGCTTCAGGATTACCTTATGTGGCAGGTGGAGTTAACGCCCTTCT
CTGACACCGATCGTGCGATTGCGACATCCATTGTCGATGCCGTTGATGACACCGGATATTTGACCGTTTCTCTGGACGAT
ATTCTTGAAAGCATCGGCGATGATGAGATCGAACTCGAGGAAATTGAAGCGGTTCTGAAGCGTATTCAGCGCTTCGACCC
GGTTGGCGTGGCCGCGAAAGATCTGCGCGACTGCTTGCTGATCCAGCTTTCTCAGTTTGCCAAAGAGACCCCCTGGATTG
AAGAAGCCCGGTTAATTATCAGCGATCATCTGGATCTGCTGGCAAACCATGACTTCCGTACACTGATGCGCGTCACCCGC
CTGAAAGAAGAAGTGTTGAAAGAAGCCGTCAATCTTATCCAGTCGCTCGATCCTCGCCCCGGCCAATCCATCCAGACCAG
CGAACCCGAATACGTTATCCCGGATGTGCTGGTCAGAAAACATAACGACCGCTGGGTTGTAGAATTGAATTCAGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCACGCAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCAGAAATGATACGCTGCTCCGTGTAAGCCGCTGTAT
TGTCGAGCAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCAGATATTGCCCAGGCCG
TCGAAATGCATGAATCAACTATTTCCCGCGTGACAACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCAATTCGTGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAATCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATCATGGTGG
CACGCCGTACTGTTGCAAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48