Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACK2UZ_RS02545 Genome accession   NZ_CP135276
Coordinates   543151..544584 (-) Length   477 a.a.
NCBI ID   WP_023295498.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain OHT403-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 538151..549584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2UZ_RS02515 (RRJ53_02530) elbB 539683..540336 (+) 654 WP_032672055.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACK2UZ_RS02520 (RRJ53_02535) mtgA 540333..541058 (+) 726 WP_315434480.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACK2UZ_RS02525 (RRJ53_02540) npr 541098..541370 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  ACK2UZ_RS02530 (RRJ53_02545) rapZ 541367..542221 (-) 855 WP_003861870.1 RNase adapter RapZ -
  ACK2UZ_RS02535 (RRJ53_02550) ptsN 542267..542758 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACK2UZ_RS02540 (RRJ53_02555) hpf 542841..543128 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  ACK2UZ_RS02545 (RRJ53_02560) rpoN/rpoN1 543151..544584 (-) 1434 WP_023295498.1 RNA polymerase factor sigma-54 Machinery gene
  ACK2UZ_RS02550 (RRJ53_02565) lptB 544632..545357 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  ACK2UZ_RS02555 (RRJ53_02570) lptA 545364..545918 (-) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACK2UZ_RS02560 (RRJ53_02575) lptC 545887..546462 (-) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  ACK2UZ_RS02565 (RRJ53_02580) kdsC 546459..547025 (-) 567 WP_315434487.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACK2UZ_RS02570 (RRJ53_02585) kdsD 547040..548026 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  ACK2UZ_RS02575 (RRJ53_02590) - 548040..549017 (-) 978 WP_003861852.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54098.98 Da        Isoelectric Point: 4.3549

>NTDB_id=886368 ACK2UZ_RS02545 WP_023295498.1 543151..544584(-) (rpoN/rpoN1) [Enterobacter hormaechei strain OHT403-4]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGNDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=886368 ACK2UZ_RS02545 WP_023295498.1 543151..544584(-) (rpoN/rpoN1) [Enterobacter hormaechei strain OHT403-4]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCAATGACGAGATTGAACTGGAAGAGATTGAAGCCGTCCTGAAACGCGTTCAGCGCTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCATGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAATGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAGGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48