Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RP314_RS18540 Genome accession   NZ_CP135199
Coordinates   3709494..3709931 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain CMML21-47     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3704494..3714931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP314_RS18525 (RP314_18525) - 3707350..3707712 (-) 363 WP_016936795.1 helix-turn-helix domain-containing protein -
  RP314_RS18530 (RP314_18530) hxlA 3707944..3708579 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  RP314_RS18535 (RP314_18535) hxlB 3708576..3709133 (+) 558 WP_077721772.1 6-phospho-3-hexuloisomerase -
  RP314_RS18540 (RP314_18540) nucA/comI 3709494..3709931 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RP314_RS18545 (RP314_18545) nin/comJ 3709953..3710351 (+) 399 WP_077721770.1 competence protein ComJ Regulator
  RP314_RS18550 (RP314_18550) - 3710370..3710600 (-) 231 Protein_3642 family 1 glycosylhydrolase -
  RP314_RS18555 (RP314_18555) - 3710814..3711089 (-) 276 WP_369719109.1 YckD family protein -
  RP314_RS18560 (RP314_18560) - 3711207..3711641 (-) 435 WP_021494203.1 RDD family protein -
  RP314_RS18565 (RP314_18565) - 3711656..3712792 (-) 1137 WP_077721769.1 zinc-dependent alcohol dehydrogenase -
  RP314_RS18570 (RP314_18570) - 3712857..3714059 (-) 1203 WP_077721768.1 GTP-binding protein -
  RP314_RS18575 (RP314_18575) - 3714204..3714332 (+) 129 Protein_3647 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=886240 RP314_RS18540 WP_003156588.1 3709494..3709931(+) (nucA/comI) [Bacillus velezensis strain CMML21-47]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=886240 RP314_RS18540 WP_003156588.1 3709494..3709931(+) (nucA/comI) [Bacillus velezensis strain CMML21-47]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641