Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   RP314_RS08035 Genome accession   NZ_CP135199
Coordinates   1530165..1530965 (+) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain CMML21-47     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 1525165..1535965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP314_RS08015 (RP314_08015) - 1525289..1526134 (+) 846 WP_077722584.1 TlyA family RNA methyltransferase -
  RP314_RS08020 (RP314_08020) ahrC 1526229..1526678 (+) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  RP314_RS08025 (RP314_08025) recN 1526710..1528440 (+) 1731 WP_077722583.1 DNA repair protein RecN Machinery gene
  RP314_RS08030 (RP314_08030) spoIVB 1528604..1529884 (+) 1281 WP_007408360.1 SpoIVB peptidase -
  RP314_RS08035 (RP314_08035) spo0A 1530165..1530965 (+) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  RP314_RS08040 (RP314_08040) alr 1531113..1532294 (-) 1182 WP_077722582.1 alanine racemase -
  RP314_RS08045 (RP314_08045) - 1532469..1532681 (-) 213 WP_014305392.1 spore germination protein -
  RP314_RS08050 (RP314_08050) - 1532808..1533020 (-) 213 WP_223883535.1 spore germination protein -
  RP314_RS08055 (RP314_08055) - 1533147..1534265 (-) 1119 WP_003153184.1 NADH-dependent flavin oxidoreductase -
  RP314_RS08060 (RP314_08060) - 1534359..1534496 (-) 138 WP_014470646.1 hypothetical protein -
  RP314_RS08065 (RP314_08065) - 1534787..1535419 (-) 633 WP_014418348.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=886209 RP314_RS08035 WP_003153177.1 1530165..1530965(+) (spo0A) [Bacillus velezensis strain CMML21-47]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=886209 RP314_RS08035 WP_003153177.1 1530165..1530965(+) (spo0A) [Bacillus velezensis strain CMML21-47]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATTGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAATTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97