Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RN333_RS02635 Genome accession   NZ_CP135147
Coordinates   536388..537821 (-) Length   477 a.a.
NCBI ID   WP_314117471.1    Uniprot ID   -
Organism   Enterobacter kobei strain DW108     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 531388..542821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN333_RS02605 (RN333_02605) elbB 532911..533564 (+) 654 WP_314117465.1 isoprenoid biosynthesis glyoxalase ElbB -
  RN333_RS02610 (RN333_02610) mtgA 533561..534292 (+) 732 WP_314117467.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  RN333_RS02615 (RN333_02615) npr 534335..534607 (-) 273 WP_088221666.1 PTS phosphocarrier protein NPr -
  RN333_RS02620 (RN333_02620) rapZ 534604..535458 (-) 855 WP_072569561.1 RNase adapter RapZ -
  RN333_RS02625 (RN333_02625) ptsN 535504..535995 (-) 492 WP_088221667.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RN333_RS02630 (RN333_02630) hpf 536078..536365 (-) 288 WP_088221668.1 ribosome hibernation promoting factor -
  RN333_RS02635 (RN333_02635) rpoN/rpoN1 536388..537821 (-) 1434 WP_314117471.1 RNA polymerase factor sigma-54 Machinery gene
  RN333_RS02640 (RN333_02640) lptB 537869..538594 (-) 726 WP_088221670.1 LPS export ABC transporter ATP-binding protein -
  RN333_RS02645 (RN333_02645) lptA 538601..539155 (-) 555 WP_072569566.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  RN333_RS02650 (RN333_02650) lptC 539124..539699 (-) 576 WP_088221671.1 LPS export ABC transporter periplasmic protein LptC -
  RN333_RS02655 (RN333_02655) kdsC 539696..540262 (-) 567 WP_088221672.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  RN333_RS02660 (RN333_02660) kdsD 540278..541264 (-) 987 WP_088221673.1 arabinose-5-phosphate isomerase KdsD -
  RN333_RS02665 (RN333_02665) - 541278..542255 (-) 978 WP_088221674.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53813.56 Da        Isoelectric Point: 4.3684

>NTDB_id=885650 RN333_RS02635 WP_314117471.1 536388..537821(-) (rpoN/rpoN1) [Enterobacter kobei strain DW108]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEIDAQPAQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDD
ILESMGDEEIGLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFAKDTPWRDEAQLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHQGRWTVELNSDSIPRLQINQHYAAMCSGSRNDADSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFDKGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTDSGGEASSTAIRALVKKMIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=885650 RN333_RS02635 WP_314117471.1 536388..537821(-) (rpoN/rpoN1) [Enterobacter kobei strain DW108]
ATGAAGCAAGGTTTGCAACTACGGCTCAGCCAACAACTGGCCATGACGCCACAATTGCAGCAGGCCATCCGCCTGCTGCA
ATTGTCTACGCTGGAATTGCAGCAAGAACTTCAGCAGGCGCTGGAAAGCAACCCCTTGCTGGAGCAAACCGATCTTCATG
AAGAGATAGACGCCCAGCCGGCTCAGGACACCGAAACCCTGGACTCTGTCGACGCCCTTGAACAGAAAGAGATGCCTGAC
GAGCTGCCACTGGATGCCAGCTGGGACGAAATCTATACCGCCGGCACGCCTTCCGGCAACGGTGTCGATTATCAGGACGA
TGAACTGCCGGTCTACCAGGGTGAAACCACCCAGTCCTTGCAGGATTACCTGATGTGGCAGGTGGAACTGACGCCCTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTTGATGCCGTAGATGACACCGGCTATCTGACCGTCTCGCTGGACGAT
ATTCTTGAAAGCATGGGCGACGAAGAGATCGGACTGGATGAAGTCGAGGCGGTACTCAAACGCGTCCAACGCTTTGATCC
GGTTGGCGTAGCCGCCCGCGATCTGCGCGACTGCCTGCTGATCCAGCTCTCCCAGTTTGCCAAAGACACCCCATGGCGCG
ACGAAGCGCAGTTGATCATCAGCGATCACCTCGATCTGCTGGCAAATCATGATTTCCGTTCACTGATGCGCGTGACGCGG
CTGAAAGAAGACGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCTCTCGATCCGCGCCCCGGTCAGTCGATCCAGACGGG
CGAACCCGAGTATGTGATCCCCGACGTGCTGGTGCGTAAGCATCAGGGTCGCTGGACGGTCGAGCTTAATTCCGACAGCA
TTCCCCGTCTGCAAATCAATCAGCACTACGCGGCTATGTGCAGCGGATCGCGCAATGACGCCGACAGTCAGTTTATCCGC
AGTAATTTGCAGGATGCCAAATGGCTGATCAAAAGCCTGGAAAGCCGTAACGACACGCTGCTGCGCGTCAGCCGCTGTAT
CGTCGAACAGCAGCAGGCCTTTTTTGATAAAGGCGAAGAATATATGAAACCGATGGTGCTGGCTGACATTGCCCAGGCCG
TCGAGATGCATGAATCGACCATTTCACGTGTTACAACCCAGAAGTACCTGCACAGCCCACGGGGCATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTAAATACCGACAGCGGCGGCGAAGCATCGTCCACCGCTATTCGCGCGCTGGTGAAGAAAAT
GATAGCCGCAGAAAACCCGGCGAAACCACTGAGTGACAGTAAGCTAACTACCCTGCTGTCCGACCAGGGTATCATGGTAG
CGCGCCGCACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482