Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   RNZ43_RS12000 Genome accession   NZ_CP135143
Coordinates   2465583..2466383 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain L33a     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2460583..2471383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RNZ43_RS11970 - 2461128..2461760 (+) 633 WP_014418348.1 helix-turn-helix domain-containing protein -
  RNZ43_RS11975 - 2462051..2462188 (+) 138 WP_014470646.1 hypothetical protein -
  RNZ43_RS11980 - 2462282..2463400 (+) 1119 WP_094247935.1 NADH-dependent flavin oxidoreductase -
  RNZ43_RS11985 - 2463527..2463739 (+) 213 WP_223883535.1 spore germination protein -
  RNZ43_RS11990 - 2463866..2464078 (+) 213 WP_003153181.1 spore germination protein -
  RNZ43_RS11995 alr 2464254..2465435 (+) 1182 WP_326375332.1 alanine racemase -
  RNZ43_RS12000 spo0A 2465583..2466383 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  RNZ43_RS12005 spoIVB 2466664..2467944 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  RNZ43_RS12010 recN 2468108..2469838 (-) 1731 WP_156735540.1 DNA repair protein RecN Machinery gene
  RNZ43_RS12015 ahrC 2469870..2470319 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  RNZ43_RS12020 - 2470414..2471259 (-) 846 WP_003153172.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=885591 RNZ43_RS12000 WP_003153177.1 2465583..2466383(-) (spo0A) [Bacillus velezensis strain L33a]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=885591 RNZ43_RS12000 WP_003153177.1 2465583..2466383(-) (spo0A) [Bacillus velezensis strain L33a]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCCGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAATTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97